Position: Home > Articles > Prediction of Eiptopes of the Major Allergen Blo t 5 from Blomia tropicalis by Using DNAStar Software
Journal of Tropical Biology
2017
(1)
107-112
利用DNAStar软件预测热带无爪螨主要致敏原Blo t 5的抗原表位
作 者：
王晓哲;肖正泮;韦双双;王大勇;裴业春
单 位：
海南大学热带农林学院动物科技学院;海南大学热带农林学院生物学院
关键词：
热带无爪螨;抗原表位;Blo t 5;DNAStar软件
摘 要：
利用DNAStar Protean软件对热带无爪螨主要致敏原Blo t 5的二级结构和表面特性进行分析,如理化性质、亲水性、表面可及性、可塑性等,预测Blo t 5蛋白的B抗原表位和T抗原表位。实验结果表明,Blo t 5蛋白的二级结构以α螺旋为主,存在6个潜在的B抗原表位位点,可能的B抗原表位区域有:18-31,39-48,56-77,86-101,105-112,117-133氨基酸残基或其附近。含有7个潜在的T细胞抗原表位区域:32-37,40-44,57-65,70-79,86-95,100-104,115-120氨基酸残基或其附近。笔者应用Protean软件预测了热带无爪螨主要致敏原Blo t 5的抗原表位,为进一步研究Blo t 5致敏原的结构与功能,研制针对热带螨的预防性和治疗性疫苗、开发相应的诊断试剂盒奠定了基础。
译 名：
Prediction of Eiptopes of the Major Allergen Blo t 5 from Blomia tropicalis by Using DNAStar Software
作 者：
WANG Xiaozhe;XIAO Zhengpan;WEI Shuangshuang;WANG Dayong;PEI Yechun;College of Animal Science and Technology,Tropical Agriculture and Forestry College,Hainan University;College of Biological Science,Tropical Agriculture and Forestry College,Hainan University;
关键词：
Blomia tropicalis;;epitope;;Blo t 5;;DNAStar software
摘 要：
The secondary structure and surface properties of Blo t 5 allergen from Blomia tropicalis,such as physical and chemical characters,hydrophilicity,surface probability,flexible regions and antigenicity,were analyzed by using the DNAStar Protean software to predict B-cell and T-cell epitopes of Blo t. 5. The secondary structure of Blo t 5 was rich in alpha helix,and the Blo t 5 contained six potential B cell epitopes located at 18-31,39-48,56-77,86-101,105-112,117-133 amino acid residues or nearby. There were seven potential T cell epitopes located at 32-37,40-44,57-65,70-79,86-95,100-104,115-120 amino acid residues or nearby. The epitopes of the major allergen Blo t 5 from B. tropicalis were predicted by using the Protean software.The results can provide a base for the studies of structure and function of the allergen Blo t 5,and development of preventive and therapeutic vaccines for Blomia tropicalis and diagnostic kits for detection of allergens induced by B. tropicalis.
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