中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 2株高致病性猪繁殖与呼吸综合征病毒分子特征分析 中国畜牧兽医 2014,41 (8) 50-56
Position: Home > Articles > Molecular Characteristics Analysis of Two Strains of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus China Animal Husbandry & Veterinary Medicine 2014,41 (8) 50-56

2株高致病性猪繁殖与呼吸综合征病毒分子特征分析

作  者：
罗成波;崔敏;孔令萍;程振涛;周碧君;毛黎红
单  位：
黔西南州疫病预防控制中心;贵州大学动物科学学院
关键词：
高致病性猪繁殖与呼吸综合征病毒;分子特征;分析;Nsp2;GP5
摘  要：
为进一步掌握黔西南州猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)流行变异趋势,对2株高致病性PRRSV(HR-PRRSV)贵州分离株GZLPS、GZSB进行Nsp2与GP5基因克隆和测序,并分析毒株分子变异特征。结果显示,2株分离株Nsp2基因有90个核苷酸不连续缺失,不同位置的36个核苷酸发生突变;GP5基因存在不同位点置换。同源性比对与系统进化树分析显示,2株分离株Nsp2与GP5基因与HP-PRRSV JXA1株及贵州省2011年以前流行毒株的核苷酸同源性在96%以上,氨基酸同源性在95%以上,但在分子变异上存在差异。结果表明黔西南州PRRSV变异方式已呈多样性。
译  名：
Molecular Characteristics Analysis of Two Strains of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus
作  者：
LUO Cheng-bo;CUI Min;KONG Ling-ping;CHENG Zhen-tao;ZHOU Bi-jun;MAO Li-hong;Center for Animal Disease Control of Qianxinan in Guizhou Province;College of Animal Science,Guizhou University;
关键词：
highly pathogenic porcine reproductive and respiratory syndrome virus;;molecular characteristics;;analysis;;Nsp2;;GP5
摘  要：
In order to find out the graph variation characteristics of porcine reproductive and respiratory syndrome virus(PRRSV)of Qianxinan in Guizhou province,the Nsp2 and GP5 genes of PRRSV isolated strain GZLPS and GZSB were cloned,sequenced and analyzed.The results showed that the Nsp2 gene of 2 isolates contained 90 nucleotides for discontinuous deletion and 36 nucleotides for mutation.Furthermore,there were different replacements of nucleotides in GP5 gene.Not only Nsp2 gene,but also GP5 gene,the nucleotide homologies were above 96% and the amino acid homologies were above 95% with strain JXA1 and isolates in Guizhou since 2011,but there were differences in molecular variation among these strains.It indicated that the PRRSV had already behaved diverse on variation style of Qianxinan in Guizhou province.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46