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当前位置: 首页 > 文章 > 大蒜MADS-box基因AsPI的克隆及表达分析 园艺学报 2017,44 (1) 69-79
Position: Home > Articles > Cloning and Expression Analysis of MADS-box gene AsPI in Garlic Acta Horticulturae Sinica 2017,44 (1) 69-79

大蒜MADS-box基因AsPI的克隆及表达分析

作  者：
徐熔;任洪杰;王海竹;娄虎;徐启江
单  位：
东北林业大学生命科学学院;杭州和壹基因科技有限公司
关键词：
大蒜;花器官发育;B功能MADS-box基因;AsPI基因
摘  要：
B类MADS-box基因在调控显花植物的花瓣和雄蕊发育过程中发挥关键作用。为初步了解大蒜花发育的分子机制,以‘阿城紫皮’大蒜(Allium sativum)的花蕾为试材克隆了AsPI(Gen Bank登录号为KY272748)。AsPI的cDNA长939 bp,开放阅读框为615 bp,编码204个氨基酸。推导的氨基酸序列显示,AsPI编码的蛋白质包含高度保守的MADS结构域(1~57氨基酸)和保守的K结构(76~140氨基酸),AsPI蛋白具有PI亚家族特有的序列特征:MADS区特征丝氨酸残基、K区高度保守序列KHExL以及C–末端的PI基序。序列比对、功能域分析及系统发育分析结果表明,AsPI属于单子叶植物B功能基因PI亚家族。半定量和实时RT-PCR检测AsPI在大蒜不同组织器官中的表达模式,结果表明,在花蕾中高丰度表达,在花茎中表达量中等,在根、假茎、嫩叶、保护叶等营养器官中表达量极低甚至不表达。
译  名：
Cloning and Expression Analysis of MADS-box gene AsPI in Garlic
作  者：
XU Rong;REN Hongjie;WANG Haizhu;LOU Hu;XU Qijiang;The College of Life Sciences,Northeast Forestry University;1 Gene,Hangzhou;
关键词：
garlic;;Allium sativum;;flower development;;B-function MADS-box genes;;AsPI gene
摘  要：
The class B genes,which belong to the MADS-box gene family,play critical roles in regulating the development of petals and stamens in flowering plants. To understand the molecular mechanisms of floral development in garlic(Allium sativum),we cloned and characterized the homologous PISTALLATA/GLOBOSA-like(PI/GLO-like)gene AsPI(Gen Bank accession number KY272748)from floral buds of garlic cultivar‘Acheng Purple Garlic'. AsPI was 939 bp in cDNA length with an open reading frame of 615 bp corresponding to 204 amino acid residues. The deduced amino acid sequence of AsPI contained a well-conserved MADS domain from amino acids 1–57 and a conserved K box from amino acids 76–140. AsPI had the serine residue and the KHExL sequence within the MADS box and the K box,respectively,a PI motif was present at the C-terminal end,all of which are characteristic of the PI subfamily. The results obtained by sequence alignment and domain analysis indicated that it might belong to PI subfamily of B function genes. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Semi-quantitative and real-time RT-PCR were done to test the expression patterns in different tissues and organs of Allium sativum. The results showed that the expression level in floral buds was the highest,whereas scape followed,little transcripts or no expression was detected in vegetative organs,such as leaf,pseudostem,protective leaf,root. This study developed the positive exploration for preliminary understanding at the molecular level on garlic floral development.

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