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当前位置: 首页 > 文章 > 稻秸对土壤细菌群落分子多态性的影响 土壤学报 2005,42 (2) 270-277
Position: Home > Articles > EFFECT OF RICE STRAW ON MOLECULAR PHYLOGENETIC DIVERSIFY OF SOILBACTERIAL COMMUNITIES Acta Pedologica Sinica 2005,42 (2) 270-277

稻秸对土壤细菌群落分子多态性的影响

作  者：
卜元卿;黄为一
单  位：
农业部农业环境微生物工程重点开放实验室 南京210095;南京农业大学生命科学学院微生物学系;农业部农业环境微生物工程重点开放实验室
关键词：
分子多态性;变性梯度凝胶电泳;稻秸;土壤细菌
摘  要：
模拟稻秸原位还田条件,分别在水稻土和红壤中添加水稻秸秆培养70d ,第0、5、2 5、4 5、70天采集土样。采用非机械破壁法直接提取水稻土和红壤细菌总DNA ,水稻土细菌总DNA经过二次纯化;红壤细菌总DNA经过一次纯化后,PCR扩增其16SrDNAV3可变区,均可获得清晰的目的条带,对扩增产物进行DGGE分析,结果显示:水稻土和红壤样品的DGGE条带增加,说明稻秸能够增加土壤细菌群落分子多态性的丰富度,随着培养期的延长,施有稻秸的处理中土壤细菌群落多态性的变化远远复杂于空白对照土壤中的细菌群落变化;同时发现在稻秸刺激下不同土壤细菌群落多态性高峰期出现时间不同
译  名：
EFFECT OF RICE STRAW ON MOLECULAR PHYLOGENETIC DIVERSIFY OF SOILBACTERIAL COMMUNITIES
作  者：
Bu Yuanqing Huang Weiyi (Microbiology Department of College of Life Science of Nanjing Agricultural University, Key Laboratory ofMicrobiological Engineering of Agricultural Environment of Agricultural Ministry, Nanjing 210095, China)$$$$
关键词：
Molecular polyphylogenetic diversify; Denaturing Gradient Gel Electrophoresis; Rice straw; Soil bacteria
摘  要：
Rice straw transformation takes place in soil but effects of rice straws on relations between soils and bacterial communities of the soils are still poorly understood. PCR and denaturing gradient gel electrophoresis (DGGE) were used to monitor development of bacterial communities in paddy soil and red soil, into which rice straw or rice straw plus biological agent was applied during incubating period, respectively. The total bacterial DNA from the two studied soils was extracted directly on D0,D5,D25,D45 and D70. The DNA from paddy soil was purified twice, but the DNA from red soil was done only once. The regions V3 of the 16S rDNA of bacteria from the two soils were amplified. DGGE profiles of the PCR productions were compared by similarity analysis. The results show simple DGGE profiles for controls of the two soils without rice straw. Rice straw was incubated in paddy soil and red soil for 70 days. DGGE profiles revealed that the composition of the bacterial community in soil with rice straw was more complex than CK during the incubating period. Meanwhile, the results show that peaks of the bacterial communities appeared at different times in the paddy soil and red soil, treated with rice straw during incubation times.

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