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Position: Home > Articles > Establishment and Modification of High Frequency Regeneration System of Perennial Ryegrass(Lolium perenne L.) Chinese Journal of Grassland 2007,29 (4) 45-49

多年生黑麦草高频再生体系的建立及优化

作  者:
蔡能;易自力;陈智勇;刘清波;蒋建雄
单  位:
湖南农业大学生物科学技术学院
关键词:
多年生黑麦草;组织培养;愈伤组织诱导;植株再生;不定芽
摘  要:
从外植体预处理方式、基本培养基、激素浓度等方面着手,对多年生黑麦草愈伤组织诱导、继代、不定芽分化及再生过程中的影响因素进行了研究,提高了再生频率。结果如下:切取胚端半粒种子,经75%乙醇处理1min,0.1%HgCl处理15min,在经无菌水清洗4~6次后接种方式较好;愈伤组织诱导培养基以MS+2,4—D 8.0 mg/L(或另添加甘露醇25 g/L)为佳;愈伤组织接种于MS+2,4—D 8.0mg/L+甘露醇25 g/L上进行1~2次继代后于NB+6—BA2.0 mg/L上进行分化;不定芽转接至MS+6—BA2.0 mg/L上进行扩繁或于1/2MS+NAA0.5mg/L+IAA0.5mg/L上进行生根。
译  名:
Establishment and Modification of High Frequency Regeneration System of Perennial Ryegrass(Lolium perenne L.)
作  者:
CAI Neng,YI Zi-li,CHEN Zhi-yong,LIU Qing-bo,JIANG Jian-xiong(College of Bioscience and Biotechnology of Hunan Agricultural University,Changsha 410128,China)
关键词:
Perennial ryegrass(Lolium perenne L.);Tissue culture;Callus induction;Plant regeneration;Adventitious bud
摘  要:
The factors influencing calli induction,subculture,differentiation and plant regeneration of Ryegrass which including explants pretreatment,basal medium and concentration of phytohormone were studied.The result showed that the half-seed to embryo,surface-sterilized by 0.1% HgCl for 15 min after dipped in 75% ethanol for 1 min could be used as explants.The calli induced on medium of MS+2,4—D8.0 mg/L(with mannitol 25 g/L or not) were best.On medium of MS+2,4-D8.0 mg/L+ mannitol 25 g/L calli were subcultured for 1 to 2 times,then cultured on medium of NB+6—BA2.0 mg/L to induce adventitious buds.Adventitious buds can be extending propagated on MS+6-BA2.0 mg/L or induced roots on medium of 1/2MS+NAA0.5mg/L+IAA0.5mg/L.

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