当前位置: 首页 > 文章 > 传统发酵永川毛霉型豆豉蛋白质提取方法比较与降解规律研究 食品科学 2013,34 (23) 180-183
Position: Home > Articles > Comparison of Methods for Protein Extraction from Traditional Fermented Yongchuan Douchi and Degradation Pattern FOOD SCIENCE 2013,34 (23) 180-183

传统发酵永川毛霉型豆豉蛋白质提取方法比较与降解规律研究

作  者:
索化夷;骞宇;阚建全
单  位:
西南大学食品科学学院;重庆市特色食品工程技术研究中心;重庆第二师范学院生物与化学工程系
关键词:
永川豆豉;蛋白质提取;方法;降解
摘  要:
比较TCA/丙酮沉淀法、酚提取法、Tris-HCl法、匀浆法4种方法对样本进行蛋白质提取的效果,研究毛霉豆豉在不同发酵阶段蛋白质的降解过程。结果表明:TCA/丙酮沉淀法适用于基于SDS-PAGE电泳的豆豉蛋白质提取。毛霉型豆豉大分子蛋白在前发酵过程中经蒸煮和毛霉分泌的蛋白酶作用后快速降解成小分子蛋白,其中蒸煮对大分子的蛋白影响最大。
译  名:
Comparison of Methods for Protein Extraction from Traditional Fermented Yongchuan Douchi and Degradation Pattern
作  者:
SUO Hua-yi;QIAN Yu;KAN Jian-quan;College of Food Science,Southwest University;Chongqing Special Food Programme and Technology Research Center;Department of Biological and Chemical Engineering,Chongqing University of Education;
关键词:
Yongchuan douchi;;protein extraction;;method;;degradation pattern
摘  要:
This study compared the effectiveness of four different methods, TCA/acetone precipitation, phenol extraction, Tris-HCl extraction and homogenization for extracting proteins from traditional fermented Yongchuan douchi(Mucor type), a soybean product originated in China. The patterns of protein degradation at different fermentation stages of Yongchuan douchi were investigated as well. SDS-PAGE showed that the TCA/acetone precipitation method was the best of the four methods. Protein macromolecules in the Mucor-type douchi were rapidly degraded into small molecules by steaming and proteases from Mucor, and the effect of steaming was more obvious.

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