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当前位置: 首页 > 文章 > 豪猪源福氏志贺菌的分离鉴定及喹诺酮类耐药基因检测 中国预防兽医学报 2019 (2) 204-208
Position: Home > Articles > Isolation and identification of Shigella flexneri from Hystrix hodgsoni and detection of quinolone resistance genes Chinese Journal of Preventive Veterinary Medicine 2019 (2) 204-208

豪猪源福氏志贺菌的分离鉴定及喹诺酮类耐药基因检测

作  者：
张鹏飞;王印;德西措姆;杨泽晓;姚学萍;罗燕;廖倡宇
单  位：
四川农业大学动物医学院;动物疫病与人类健康四川省重点实验室;西藏昌都市左贡县畜牧站
关键词：
豪猪;福氏志贺菌;分离鉴定;人兽共患病;喹诺酮耐药
摘  要：
为调查四川省某规模化豪猪养殖场中豪猪大面积腹泻死亡的原因,本研究采集病死豪猪的肠道内容物和肺脏等病料样品进行细菌的分离纯化,通过培养特性观察、革兰染色镜检、生化鉴定、16S rRNA基因的克隆测序进行鉴定,并对分离菌株进行致病性、耐药性分析,对其喹诺酮类耐药基因进行PCR检测并测序分析。结果显示,分离得到1株革兰阴性菌,该分离株符合福氏志贺菌的培养特性和生化特性,并且其16S rRNA基因序列与福氏志贺菌该基因序列的同源性为99%;对小鼠具有较强致病性;药敏试验结果显示分离株具有多重耐药性,仅对丁胺卡那敏感,对喹诺酮类、四环素类、头孢类、氨基糖苷类、青霉素类和氯霉素类药物均表现为耐药;分离株的DNA促旋酶Ⅱ和拓扑异构酶Ⅳ的喹诺酮耐药决定区均有突变,且携带有质粒介导的喹诺酮耐药基因qnr A和qnr S。本研究是国内首次关于豪猪源福氏志贺菌病的报道,为豪猪养殖中该细菌性疾病的诊断防治等相关研究提供参考。
译  名：
Isolation and identification of Shigella flexneri from Hystrix hodgsoni and detection of quinolone resistance genes
作  者：
ZHANG Peng-fei;WANG Yin;DE Xi Cuo-mu;YANG Ze-xiao;YAO Xue-ping;LUO Yan;LIAO Chang-yu;College of Veterinary medicine, Sichuan Agricultural University;Key Laboratory of Animal Disease and Human of Sichuan Province;Tibet Changdu Zuogong County Animal Husbandry Station;
单  位：
ZHANG Peng-fei%WANG Yin%DE Xi Cuo-mu%YANG Ze-xiao%YAO Xue-ping%LUO Yan%LIAO Chang-yu%College of Veterinary medicine, Sichuan Agricultural University%Key Laboratory of Animal Disease and Human of Sichuan Province%Tibet Changdu Zuogong County Animal Husbandry Station
关键词：
Hystrix hodgsoni;;Shigella flexneri;;isolation and identification;;zoonosis;;quinolone resistance
摘  要：
The aim of this study was to explore the cause of the death of Hystrix hodgsoni in a large-scale farm in Sichuan province. Intestine contents and lungs of dead H.hodgsoni were collected for bacterial isolation. The staining and culture characteristics of the isolate were observed and identified by biochemical tests. The 16S rRNA gene of isolate was amplified and cloned for sequencing. And mice infection test and drug sensitive test was conducted to analyze pathogenicity and drug resistance of isolate. Moreover, mutations within quinolone resistance gene of the isolate were identified by PCR. The results showed, the culture and biochemical characteristics of the isolate was Shigella flexneri. And the results of the 16S rRNA sequencing of the isolate showed 99% homology with S.flexneri. In addition, the isolate had pathogenicity to mice. The results of antibiotic sensitivity test showed that it was sensitive to amikacin but resistance to quinolone, tetracycline, cephalosporin, aminoglycoside,benzylpenicillin and chloramphenicol. Furthermore, the isolate had mutations in the quinolone resistance determining regions(QRDR) of gyr A and par C and carry plasmid-mediated quinolone resistance genes of qnr A and qnr S. This study is the first report of S.flexneri infestation on the H.hodgsoni breeding industry in China and provides references for the disease prevention and drug treatment of associated Shigella disease.

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