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Position: Home > Articles > Quick Isolation of Total DNA from Dichondra Repens Forst Journal of Sichuan Agricultural University 2004,22 (4) 105-106+110

马蹄金总DNA的快速提取

作  者:
刘明秀;张新全;白史且;马啸
单  位:
四川省草原研究所;四川农业大学动物科技学院
关键词:
马蹄金;DNA提取
摘  要:
利用E .Z .N .A微量植物DNA提取试剂盒 ,在 1h内可快速可靠地从马蹄金 (DichondrarepensForst)植物样本或组织中提取纯度较高的总DNA。这套系统利用速度和多变的旋转技术来结合DNA吸附旋转柱中基质的核酸内容物 ,从而除去植物组织中的多糖、酚化合物、酵素抑制剂。所得DNA产物纯度高可直接用于PCR ,RAPD、AFLP等各种下游分析技术
译  名:
Quick Isolation of Total DNA from Dichondra Repens Forst
作  者:
LIU Ming-xiu~1, ZHANG Xin-quan~(1), BAI Shi-qie~2, MA Xiao~1 (1.College of Animal and Technology, Sichuan Agricultural University, Yaan 625014, Sichuan, China; 2.Sichuan Provincial Grassland Research Institute, Chengdu Xipu 611731, Sichuan, China)
关键词:
Dichondra repens Forst; DNA isolation
摘  要:
By using the E.Z.N.A Plant Miniprep Kit, high-quality total celluar DNA can be rapidly and reliably isolated from Dichondra repens Forst of species and tissues. Up to 100?mg of wet tissue (or 30?mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acidbinbing properties of HiBind matrix with the speed and versatility polysaccharides phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, RAPD, AFLP and hybridization techniques.

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