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Position: Home > Articles > The Comparisons and the Analyses of Changes in Phospholipids from Leaf of Ivy Growing in Different Temperature Conditions Acta Horticulturae Sinica 2003,30 (4) 431-435

不同温度条件下常春藤叶片磷脂变化的比较分析

作  者:
沈漫
单  位:
北京农学院植物科学技术系
关键词:
常春藤;磷脂;脂肪酸;膜脂流动性;抗寒性
摘  要:
以栽培在露地和温室的常春藤 (Hederanepalensisvar.sinensis)为材料 ,运用荧光偏振、薄层层析扫描、气相色谱等技术测定叶片磷脂流动性、磷脂及其脂肪酸组成配比。结果表明 ,与温室栽培相比 ,露地栽培的常春藤具有较高的不饱和磷脂和不饱和脂肪酸含量。在低温胁迫下 ,磷脂酰胆碱 (PC)的不饱和脂肪酸水平是影响膜脂流动性的主要因素。通过对不同温度条件下生长的常春藤的磷脂流动性、磷脂和脂肪酸组成的差异性分析 ,从膜脂组成与结构的角度探讨了常绿阔叶植物的抗寒机理
译  名:
The Comparisons and the Analyses of Changes in Phospholipids from Leaf of Ivy Growing in Different Temperature Conditions
作  者:
Shen Man (Department of Plant Science and Technology,Beijing Agriculture College,Beijing 102206,China)
关键词:
Hedera nepalensis; Phospholipids;Fatty acids;Membrane lipid fluidity;Cold resistance
摘  要:
Selected ivy( Hedera nepalensis var. sinensis )growing in open field and greenhouse separately as the experimental material,the fluidity and the composition of fatty acids in phospholipids isolated from the leaves were determined by fluorescence polarization,scanning thin layer chromatography and gas chromatography methods.The results showed that ivy growing in open field in January possessed the higher level of unsaturated phospholipids(PC and PE)and fatty acids(16∶1+18∶1+18∶2+18∶3)than that growing in greenhouse.For ivy growing in open field,phosphatidyl choline(PC)was the main factor to influence its membrane lipid fluidity during it adapted to the low temperature stress.By analyzing the differences of fluidity and composition of phospholipids from the leaves of ivy growing in different temperature conditions,the mechanism of the cold resistance of evergreen broad leaved plant in terms of the composition and the structure of membrane lipids were discussed.

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