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当前位置: 首页 > 文章 > Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure 农业科学学报 (英文) 2014,13 (6)
Position: Home > Articles > Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure Journal of Integrative Agriculture 2014,13 (6)

Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure

作  者：
Ke Xin;Wang Chun-yong;Li Run-dong;Zhang Yun
单  位：
Shenyang Agr Univ, Coll Land & Environm, Shenyang 110161, Peoples R China;Shenyang Aerosp Univ, Coll Energy & Environm, Shenyang 110136, Peoples R China
关键词：
oxytetracycline;anaerobic digestion stages (BDS, DMS and FDS);PCR-DGGE;bacterial community;archaeal community
摘  要：
The effects of different concentrations of oxytetracycline (OTC) on the dynamics of bacterial and archaeal communities during the mesophilic anaerobic digestion (37 degrees C) of cow manure were investigated. Before anaerobic digestion, OTC was added to digesters at concentrations of 20, 50, and 80 mg L-1, respectively. Compared with no-antibiotic control, all methane productions underwent different levels of inhibition at different concentrations of OTC. Changes in the bacterial and archaeal communities were discussed by using PCR-denaturing gradient gel electrophoresis (DGGE). Results showed that OTC affected the richness and diversity of bacterial and archaeal communities. The bacterial genus Flavobacterium and an uncultured bacterium (JN256083.1) were detected throughout the entire process of anaerobic digestion and seemed to be the functional bacteria. Methanobrevibacter boviskoreani and an uncultured archaeon (FJ230982.1) dominated the archaeal communities during anaerobic digestion. These microorganisms may have high resistance to OTC and may play vital roles in methane production.

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