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Position: Home > Articles > Identification of Strains of Barley Yellow Dwarf Virus in Harbin Area Heilongjiang Agricultural Sciences 1996 (6) 1-3

哈尔滨地区大麦黄矮病毒株系鉴定

作  者:
刘艳;钱幼亭;梁影屏;苗洪芹
单  位:
中国农业科学院植物保护研究所
关键词:
哈尔滨地区;大麦黄矮病毒株系;酶联免疫吸附
摘  要:
从黑龙江省哈尔滨地区田间采集典型小麦黄矮病株25份,用无毒麦二叉蚜(Shizaphis graminum)、麦长管蚜(Macrosiphum avenae)、禾谷缢管蚜(Rhopalosiphum-padi)传毒,经生物学分离并用BYDV—GAV、PAV、RPV、SGY四种抗血清进行酶联免疫吸附(即ELISA法)测定。结果表明:25份标样中由麦长管蚜(Ma)和麦二叉蚜(Sg)传播的有22份,与GAV抗血清有强反应,而与PAV、RPV、SGV抗血清无反应。表明GAV株系是哈尔滨地区大麦黄矮病毒的主要株系。
译  名:
Identification of Strains of Barley Yellow Dwarf Virus in Harbin Area
作  者:
Liu Yan Qian Youting Liang Yingping Miao Hongqin (Institute of plant Protection, Chinese Academy of Agricultural Sciences)
关键词:
Harbin area, Barley yellow dwarf virus strain,ELISA
摘  要:
Samples of 25 plants of wheat with symptoms of barley yellow dwarf were collected from Harbin area, Heilongjiang province. Each sample was tested by enzyme-linked immunosor-bent assays (ELISA) using antisera for GAV,PAV,RPV,SGV and identifed by a series of biological experiments involving comparative transmission by Schizaphis graminum, Rhopalosiphum Padi and Macrosiphum avenae. Results indicated that 22 of 25 smples were transmitted non-specifically by S. avenae and S. graminum, and reacted very strongly with GAV-globulin and not at all with PAV,RPV and SGV globulins. These results pionted that GAV is the major strain of Barley Yellow Dwarf Virus in Harbin area.

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