关键词：
麻叶千里光;提取;黄酮;薄层扫描法;紫外分光光度法
摘 要：
目的:以麻叶千里光为原料,采用索氏提取和微波提取相结合的方法提取黄酮并测定其提取率。方法:通过对索氏提取时间、微波提取时间、微波提取功率单因素试验确定最佳单因素水平并做正交试验,确定以甲醇为提取剂提取麻叶千里光中黄酮类化合物的最佳工艺条件。然后采用紫外分光光度法和薄层扫描法测定黄酮提取率。结果:最佳提取工艺为索氏提取时间2h、微波提取时间150s、微波功率80W。紫外分光光度法测定的麻叶千里光中总黄酮提取率为0.543%、回收率为92.49%、精密度为4.744%,薄层扫描法测定的麻叶千里光中总黄酮含量为0.783%、回收率为99.20%、精密度0.380%。结论:薄层扫描法测定麻叶千里光中总黄酮提取率方法准确、快速。
译 名：
Determination of Total Flavonoids in Senecio cannabifolius Less
作 者：
GAO Shu-yun,CHENG Xi (Xuzhou Institute of Technology, Xuzhou 221008, China)
关键词：
Senecio cannabifolius Less;extraction;flavonoids;TLC;UV
摘 要：
In this study, total flavonoids was prepared from raw materials of Senecio cannabifolius Less by using soxhlet extraction and microwave extraction. A single-factor test and an orthogonal experiment were employed to investigate the effect of extraction time and the microwave power on the yields of flavonoids. The optimal extracting conditions were obtained as follows: 2 h of extraction time for soxhlet and 150 s for microwave and the microwave power of 80 W. The Senecio cannabifolius Less contained 0.543% of flavonoids by UV method and the recovery of this analysis was 92.49% and 4.744% for precision while the content of flavonids by TLCS was 0.783% and the recovery was 99.20% and 0.380% for precision test.
