Isolation and Identification of Equine Rhinopneumonitis Virus from Przewalskii Horses in Xinjiang

Shan Wenlu Du Jian Chao Qunfang Turxun Kelimu Ran Duoliang Ren Youcai Jia Liqun Shi Yuanxinag (College of Animal Science,Xinjiang Agricultural University,Urumqi 830052)

Przewalskii Horse equine rhinopneumonitis virus isolation and identifications

We had isolated a virus strain from the sample of Przewalskii Horses at XinJiang Przewalskii Horse Breeding Centre in Jimsar County,Xinjiang Uygor Automous Region.The virus had been identified.It was continually inoculated 6 generations onto hamster kindey cells (BHK 21) and induced typical cytopathogenic effect (CPE).Culturing the virus on chicken embryonic fibroblast cells,sucking guinea pig kindey and testicle cells,the CPE could be seen but there was difference.The typical equine rhionopneumonitis virus particles (Like Herpesvirus particles) could be found under electromicroscope.The isolated virus was sensitive to 5 iod 2 dexoy uridine,chloroform,ether etc.It lost activity in pH3 and was inactivated for 30 minutes at 56℃.The virus could be neutralized by the standard positive serum to equine rhinopneumonitis virus.The sucking guinea pigs were injected with the isolates and typical lethal hepatitis could be observed.All of the evidences indicated that the isolated virus was equine rhinopneumonitis virus(ERV),and it was named PH93 1 strain of equine rhinopneumonitis virus.