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当前位置: 首页 > 文章 > 一株好氧反硝化细菌的分离鉴定及其脱氮性能研究 江西农业大学学报 2024 (3) 809-818
Position: Home > Articles > Isolation and identification of an aerobic denitrifying bacteria and its denitrification performance Acta Agriculturae Universitatis Jiangxiensis 2024 (3) 809-818

一株好氧反硝化细菌的分离鉴定及其脱氮性能研究

作  者：
邱梦瑶;苏应兵;周燚
单  位：
;长江大学动物科学技术学院
关键词：
好氧反硝化;斯图泽里单胞菌;脱氮性能;环境因子
摘  要：
[目的]旨在为净化富氮养殖水体筛选一株高效的好氧反硝化细菌.[方法]从虾池底泥和水样中通过微生物富集分离的方法筛选得到一株好氧反硝化细菌,并根据形态学特征和16S rDNA序列分析的结果,对菌种进行初步鉴定和利用PCR技术对反硝化功能基因进行扩增,通过单因素试验对菌株ZY-F8的脱氮条件(碳源、温度、pH)进行优化,最后研究菌株在富氮养殖水中的反硝化能力.[结果]从虾池中分离出的好氧反硝化细菌(ZY-F8)初步鉴定为斯图泽里单胞菌(Stutzerimonas stutzeri),功能基因nirS和napA在菌株ZY-F8体中被成功扩增出,说明菌株ZY-F8能在有氧条件下进行脱氮作用.菌株ZY-F8在温度为30℃、碳源为柠檬酸钠、初始pH为7.0的培养条件下生长和脱氮效果最佳,对硝氮的去除率高达99%以上,亚硝态氮的累积量均在0.0025 mg/L左右.[结论]菌株ZY-F8在处理养殖污水中表现出良好的反硝化性能,在脱氮工艺中具有良好的应用前景.
译  名：
Isolation and identification of an aerobic denitrifying bacteria and its denitrification performance
单  位：
College of Animal Science and Technology,Yangtze University,Jingzhou,Hubei 434023,China;College of Agriculture,Yangtze University,Jingzhou,Hubei 434023,China
关键词：
aerobic denitrification%stutzerimonas%nitrogen removal performance%environmental factors
摘  要：
[Objective]In order to purify nitrogen-rich aquaculture water,an efficient aerobic denitrifying bacteria was screened out.[Method]An aerobic denitrifying bacterium was screened from shrimp pond sediment and water samples by microbial enrichment and separation.The results were compared according to physiological and biochemical characteristics and 16S rDNA sequencing,the strain was identified and the key denitrification genes wereamplified.Nitrogen removal performance of strains under different environmental factors(temperature,carbon source,initial pH)was optimized.Finally,the nitrogen removal abilityof this strain in nitrogen-rich wastewater was investigated.[Result]The aerobic denitrifying bacteria(ZY-F8)isolated from shrimp ponds was initially identified as Stutzerimonas stutzeri.The denitrification enzyme genes nirS and napA were detected in the expression of the strain,indicating that the strain hasdenitrification ability in aerobic environment.In the single factor experiment,it was concluded that the strain had the best denitrification effect under the culture conditions of temperature of 30℃,carbon source of sodium citrate and initial pH of 7.0.The removal rate of nitrate nitrogen reached more than 99%,and the cumulative content of nitrite nitrogen was about 0.0025 mg/L.[Conclusion]The strain ZY-F8 showed good denitrification performance in the treatment ofaquaculture wastewater,and had a good application prospect in the denitrification process.

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