Optimization of extraction process of Qingjie mixture

ZHANG Qiaomei;TIAN Lang;XU Wali;HUANG Lanxiu;CHEN Zhenwei;WU Baoqing;Animal Husbandry and Fishery Research Center of Guangdong Haid Group Co.,LTD.;Guangzhou Heshengtang Animal Pharmaceutical Co.,LTD.;

Qingjie mixture;;baicalin;;extraction process;;single factor test;;orthogonal test;;HPLC

In order to optimize the extraction process of Qingjie mixture,and extract the effective components in the medicine,single factor experiment was used to determine the effect of different factors on extraction process and determine the factors and levels of the orthogonal experiment,orthogonal experiment was carried out by using high performance liquid chromatography(HPLC)to detect the content of baicalin,baicalin content and dry extract yield as the comprehensive evaluation index,weighted scoring method was adopted to improve the comprehensive score and screen the best extraction technology of Qingjie mixture. The results showed that the optimal extraction conditions were as follows:solid-liquid ratio of 1∶15(g∶m L),extraction time of 1 h,extraction times of 2 times,linear regression equation of baicalin was y =37 499 x-112 622(R2=0.999 6),and a good linear relationship was found between7.81-500 μg·m L~(-1) of baicalin reference. The results showed that the optimized extraction process was stable and feasible,which provided a theoretical basis for industrial production.