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牛磺胆酸对小鼠腹腔巨噬细胞Gsα基因表达的影响

作  者:
郭文瑞;毛伟;关红;李培锋
单  位:
内蒙古农业大学兽医学院
关键词:
牛磺胆酸;刺激性G蛋白α;荧光定量PCR;腹腔巨噬细胞
摘  要:
本试验旨在探讨牛磺胆酸(taurocholate acid,TCA)对小鼠腹腔巨噬细胞刺激性G蛋白α(Gsα)基因表达的影响,为阐明TCA对小鼠腹腔巨噬细胞的作用机制提供线索。采用荧光定量PCR技术检测TCA对小鼠腹腔巨噬细胞GsαmRNA表达的影响。试验结果显示,高(15μg/mL)、中(1.5μg/mL)和低剂量(0.15μg/mL)的TCA作用组小鼠腹腔巨噬细胞GsαmRNA的表达均显著高于对照组和LPS刺激组(P<0.05)。结果表明,TCA能显著促进小鼠腹腔巨噬细胞GsαmRNA的表达。
译  名:
Effect of TCA on Gsα Expression in Peritoneal Macrophage in Mouses
作  者:
GUO Wen-rui1,2,MAO Wei1,2,GUAN Hong1,2,LI Pei-feng1,2 (1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2.Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease,Ministry of Agriculture,Hohhot 010018,China)
关键词:
taurocholate acid;Gsα;Real-time fluorescent quantitative PCR;peritoneal macrophage
摘  要:
The assay was aimed to explore the effect of taurocholate acid(TCA) on Gsα expression in peritoneal macrophage in mouses,and provide clues for explaining the function mechanism of TCA on mouse peritoneal macrophages.The peritoneal macrophages of mouse were isolated and cultured.The effect of TCA on the expression of Gsα mRNA was examined quantitatively by Real-time fluorescent quantitative PCR.TCA(15,1.5 and 0.15 μg/mL) decreased significantly the expression of Gsα mRNA in peritoneal macrophage in mouses(P<0.05),compared with that in control group and LPS group.The results showed that TCA could significantly promote the expression of Gsα mRNA in peritoneal macrophage in mouses.

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