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当前位置: 首页 > 文章 > 棉铃虫C型凝集素抑制多角体病毒体内增殖 植物保护学报 2014,41 (4) 468-473
Position: Home > Articles > A C-type lectin from the cotton bollworm inhibiting NPV proliferation in vivo Journal of Plant Protection 2014,41 (4) 468-473

棉铃虫C型凝集素抑制多角体病毒体内增殖

作  者：
柴连琴;何世斌;王楠;孟景会;苗迎春;于威
单  位：
河南大学生命科学学院;广东嘉应制药股份有限公司;通许县农林局
关键词：
棉铃虫;C型凝集素;RNA干扰;棉铃虫核型多角体病毒
摘  要：
为探究C型凝集素(C-type lectin)在病毒传播中的可能作用,采用半定量RT-PCR法检测了棉铃虫Helicoverpa armigera不同C型凝集素在核型多角体病毒(nuclearpolyhedrosis virus,NPV)感染后的表达变化,并通过real-time qPCR和RNA干扰技术研究了NPV在棉铃虫体内的增殖状况及对棉铃虫致死率的影响。结果显示,在感染NPV后,棉铃虫血细胞C型凝集素Ha-lectin1和Halectin5基因表达变化明显,其中Ha-lectin1在血细胞和脂肪体中的表达显著上调;注射dsRNA后,Ha-lectin1基因相对于actin的表达量均值由0.19下降到0.04,下降幅度达79%,显示Ha-lectin1基因被成功干扰。随着Ha-lectin1基因的沉默,病毒滴度由0.0015快速升高到1.95,棉铃虫的死亡率也随之增加到97.7%。表明C型凝集素能够抑制NPV在棉铃虫体内的增殖。
译  名：
A C-type lectin from the cotton bollworm inhibiting NPV proliferation in vivo
作  者：
Chai Lianqin;He Shibin;Wang Nan;Meng Jinghui;Miao Yingchun;Yu Wei;State Key Laboratory of Cotton Biology,College of Life Sciences,Henan University;Guangdong Jiaying Pharmaceutical Company Limited;Bureau of Agriculture and Forestry of Tongxu County;
关键词：
Helicoverpa armigera;;C-type lectin;;RNAi;;HaNPV
摘  要：
In order to explore the possible role of C-type lectin in viral dissemination,the expression level of the cotton bollworm C-type lectins was detected after nuclearpolyhedrosis virus( NPV) challenge;meanwhile,real-time qPCR and RNAi were used to study the change in proliferation of NPV and mortality of the cotton bollworm. The results showed that the expression of hemocytes Ha-lectin1 and Halectin5 changed obviously after NPV infection,and Ha-lectin1 was significantly up-regulated in hemocytes and fat body. The mean of Ha-lectin1 relative to actin expression decreased from 0. 19 to 0. 04 after dsRNA injection, dropping by 79%, which indicated that Ha-lectin1 expression was successfully interfered. With the silence of hemocyte Ha-lectin1,the virus titers increased rapidly from 0. 0015 to1. 95,accompanied with a 97. 7% increase of mortality in the cotton bollworm. The results suggested that C-type lectin could inhibit NPV proliferation in Helicoverpa armigera.

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