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Position: Home > Articles > Molecular Identification of Suspected Claw Sheath from Ursus thibetanus in Chebaling National Nature Reserve,Guangdong Province Journal of Northeast Forestry University 2011,39 (11) 105-107

广东车八岭自然保护区疑似黑熊爪鞘的分子鉴定

作  者:
廖丽晶;金崑;胡慧建;张于光;张亮
单  位:
华南濒危动物研究所;中国林业科学研究院森林生态环境与保护研究所
关键词:
亚洲黑熊;线粒体Cytb基因;物种鉴定;车八岭国家级自然保护区
摘  要:
利用食肉目线粒体Cyt b基因的特异性引物对2010年4月在广东车八岭自然保护区内发现的爪鞘进行了分子鉴定。所测序列与亚洲黑熊(Ursus thibetanus)的同源性最高,达97.2%~99.1%,确定爪鞘来自于亚洲黑熊,为近20 a来首次在车八岭保护区发现的亚洲黑熊存在痕迹。
译  名:
Molecular Identification of Suspected Claw Sheath from Ursus thibetanus in Chebaling National Nature Reserve,Guangdong Province
作  者:
Liao Lijing,Jin Kun(Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,P.R.China);Hu Huijian(South China Institute of Endangered Animals);Zhang Yuguang(Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry);Zhang Liang(South China Institute of Endangered Animals)
关键词:
Ursus thibetanus;Cyt b gene;Species identification;Chebaling national nature reserve
摘  要:
DNA was directly extracted from a claw sheath found in Chebaling National Nature Reserve,then the mtDNA Cyt b gene fragment was amplified and sequenced.Sequences were aligned with the GenBank by BLAST software,which showed the highest homology to the sequences from the black bear(Ursus thibetanus),reaching 97.2%-99.1%.The claw sheath was identified from U.thibetanus.It is the first time we have discovered trace of U.thibetanus appeared in Chebaling National Nature Reserve in recent 20 years.

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