中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > A型魏氏梭菌抗毒素的检测 江苏农业学报 1988 (3) 38-42
Position: Home > Articles > Studies on Detection of Type A Clostridium welchii ( C. perfringens ) Antitoxins Jiangsu Journal of Agricultural Sciences 1988 (3) 38-42

A型魏氏梭菌抗毒素的检测

作  者：
郭明章;何正礼;董亚芳
单  位：
江苏省农业科学院畜牧兽医研究所
关键词：
家兔;A型魏氏梭菌;抗毒素;间接血凝试验
摘  要：
建立了检测A型魏氏梭菌抗毒素的间接血凝试验（IHA）,可用于家兔A型魏氏梭菌下痢的诊断、流行病学调查和免疫监测。IHA与小白鼠中和试验检测血清样品,符合率为88.9％.毒素致敏红细胞易于保存,在4℃保存6个月或在室温保存40天,均不失去其效力。测定A型魏氏梭菌苗免疫家兔产生抗毒素的动态变化,表明抗毒素的产生与机体免疫力的发生呈平行关系。免疫21天后,家兔可产生较强的免疫力,能维持5─6个月。
译  名：
Studies on Detection of Type A Clostridium welchii ( C. perfringens ) Antitoxins
作  者：
Guo Mingzhang, He Zhengli and Dong Yafang (Institute of Animal Husbandry & Veterinary Science, Jiangsu Academy of Agricultural Sciences)
关键词：
Rabbit; Type A Closiridium welchii; Antitoxin; Detection;IHA
摘  要：
Indirect hemagglutination test (IHA) was established for detection of antitoxins against type A Clostridium welchii antitoxins. In detecting rabbit serum antitoxins, IHA and neutralization test (NT) with mice showed good correlations. 119 rabbit sera were detected for type A C. welchii antitoxins by IHA, the positive rate being 42.0%(50/119). The conforming rate was 88.9% between IHA and NT in detecting rabbit sera. Comparatively, IHA was more rapid and simple, and could be used in clinical diagnosis, epide- miological survey and immunosurveillance. The toxin-sensitized sheep erythrocytes stabilized by pyruvic aldehyde and formeldehyde could be stored at 4℃ for more than 6 months and at room temperature for at least 40 days without significant decline in their sensitivity. The dynamics of production of antitoxins in rabbits immunized with type A C. welchii bacterin were observed. Antitoxins appeared in sera 5 days after inoculation of the bacterin, reached the peak after one month, and gradually decreased after 4-5 months. Twenty-one days after vaccination, the rabbit developed strong immunity and maintained for 4-5 months. It was shown that the antitoxin level of serum corresponded to the immunity of rabbit.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46