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当前位置: 首页 > 文章 > 流感病毒感染的p53+/+小鼠和p53-/-小鼠肺组织中细胞凋亡相关基因表达变化的分析 中国兽医科学 2012 (8) 809-813
Position: Home > Articles > Differential expression of apoptosis-related genes in lungs between p53+/+ mice and p53-/-mice infected with influenza A virus Chinese Veterinary Science 2012 (8) 809-813

流感病毒感染的p53+/+小鼠和p53-/-小鼠肺组织中细胞凋亡相关基因表达变化的分析

作  者：
晏文君;史子学;魏建超;朱紫祥;邓绪芳
单  位：
中国农业科学院上海兽医研究所;军事医学科学院军事兽医研究所
关键词：
流感病毒;p53;细胞凋亡;基因芯片技术
摘  要：
用流感病毒PR8株感染8～10周龄p53+/+小鼠和p53-/-小鼠,分别在病毒感染后第3天和第6天采取小鼠肺组织,用基因芯片技术检测肺组织中细胞凋亡相关基因的表达水平,并对差异基因进行分析。结果显示,在病毒感染后第6天,p53+/+小鼠与p53-/-小鼠相比,促进细胞凋亡的基因表达明显上调,而抑制细胞凋亡的基因表达下调。比较caspase家族基因表达变化的结果显示,caspase 3和caspase 7的表达明显上调,而caspase 1和caspase 4的表达有所下调。此外,参与调控细胞凋亡的p53靶基因的表达也有明显的变化。研究结果表明,p53可能通过引起细胞凋亡而起到抗流感病毒的作用。
译  名：
Differential expression of apoptosis-related genes in lungs between p53+/+ mice and p53-/-mice infected with influenza A virus
作  者：
YAN Wen-jun1,SHI Zi-xue1,WEI Jian-chao1,ZHU Zi-xiang1,DENG Xu-fang1,SHAO Dong-hua1, WNAG Shao-hui1,LI Bei-bei1,WU Kai-bao2,MA Zhi-yong1(1.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China; 2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)
关键词：
influenza virus;p53;cell apoptosis;oligonucleotide micro-array
摘  要：
To analyze the difference in expression of apoptosis-related genes in the presence and absence of p53 for exploring mechanism of p53 antiviral activity,p53+/+ mice and p53-/-mice were infected with influenza A virus PR8 strain.The expressions of apoptosis-related genes in lungs were detected by oligonucleotide microarrays on days 3 and 6 post-infection,with the changes of mRNA expression profile analyzed.Result showed that the expression of positively-regulated apoptosis genes was up-regulated in the p53+/+ mice compared with the p53-/-mice,while the expression of negatively-regulated apoptosis genes was down-regulated,on day 6 post-infection.The expression of caspase 3 and caspase 7 were obviously up-regulated,while caspase 1 and caspase 4 were down-regulated.Some p53 targeting genes that regulates apoptosis expressed at a significant level in the p53+/+ mice compared with that in the p53-/-mice.The results implied that p53 was involved in regulation of apoptosis that has a role in antiviral response.

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