Zhuqing Dai;Meimei Nie;Ye Chen;Jiangfeng Song;Yayuan Xu;Zhongyuan Zhang;Guodong Zhang;Shumo Yan;Xing Zhang;Dajing Li

Institute;Jiangsu;Aland

LUTEIN;STEVIOSIDE;Antioxidant;Human;retinal;pigment;epithelial;cell;Mechanism

In order to improve the bioavailability of lutein(LUT),a novel lutein-stevio side nanoparticle(LUT-STE)were prepared previously,but the information about LUT-STE on protecting of eye health was limited.This study investigated the effect of LUT-STE on antioxidant activity of H₂O₂-induced human retinal pigment epithelial(ARPE)cells.LUT and LUT-STE(final concentration of 5μg/mL)significantly enhanced cell viability from(74.84±5.10)%to(81.92±10.01)%(LUT)and(89.33±4.34)%(LUT-STE),and inhibited the cell apoptosis(P<0.05).After pretreatment with LUT-STE in ARPE cells,the levels of superoxide dismutase(SOD),catalase(CAT)and glutathion peroxidase(GSH-Px)in ARPE cells were significantly increased(P<0.05),the contents of reactive oxygen species(ROS)and malondialdehyde(MDA)were decreased.In addition,the vascular endothelial growth factor(VEGF)levels were inhibited by 13.61%and 17.39%,respectively,pretreatment with LUT and LUT-STE.Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H₂O₂-induced apoptosis.In summary,the novel delivery LUT-STE had more pronounced inhibitory effect on H₂O₂-induced damage in human ARPE cells.