Position: Home > Articles > Purification and extraction of pig lung trypsin Inhibitor and analysis part of the physical and chemical nature
Guangdong Agricultural Sciences
2010,37
(7)
167-169
猪肺抑肽酶分离纯化及部分性质分析
作 者：
丁鸿;林延填;谭竹钧;韩雅莉
单 位：
广东工业大学轻工化工学院
关键词：
Sepharose4B;亲和层析;抑肽酶;猪肺;理化性质
摘 要：
以新鲜猪肺为材料,Sepharose4B偶联胰蛋白酶作为亲和层析介质,从猪肺中分离纯化高比活猪肺抑肽酶。结果表明,猪肺的抑肽酶含量为1.86mg/kg,酶比活力达4593U/mg;猪肺抑肽酶耐热性好、对pH敏感性低,在280nm处有最大光吸收,对胰蛋白酶有很强的抑制作用。
译 名：
Purification and extraction of pig lung trypsin Inhibitor and analysis part of the physical and chemical nature
作 者：
DING Hong1,2, Lin Yan-tian1, TAN Zhu-jun1, Han Ya-li1 (1.Faculty of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006,China; 2. Department of Biology, Jieyang College, Jieyang, 522000,China)
关键词：
Sepharose4B; affinity chromatography; aprotinin;pig lung; physical and chemical nature
摘 要：
The aprotintin with high activity was extracted and purified by affinity chromatography, based on crosslinking sepharose4B immobilizing trypsin.The results showed that the aprotinin yield was 1.86 mg/kg,the special activity of aprotinin reached 4 593 U/mg.The aprotinin was not only high heat-resistance,but also low pH sensitivity. And it had light absorption at 280 nm and strong inhibition to trypsin.
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