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当前位置: 首页 > 文章 > 大白菜脱春化效果及脱春化前后DNA甲基化分析 中国蔬菜 2013,1 (8) 26-31
Position: Home > Articles > Effects of Chinese Cabbage Devernalization and DNA Methylation Analysis before and after Devernalization China Vegetables 2013,1 (8) 26-31

大白菜脱春化效果及脱春化前后DNA甲基化分析

作  者：
王晓丽;赵勇;张耀伟
单  位：
黑龙江省经济作物技术指导站;农业部东北地区园艺作物生物学与种质创制重点实验室;东北农业大学园艺学院
关键词：
大白菜;脱春化;DNA;甲基化
摘  要：
针对春大白菜极易经历低温春化而先期抽薹的现象,以大白菜一代杂种阳春为试材,在光照培养箱条件下进行绿体脱春化、种子脱春化及在温室条件下进行绿体脱春化研究。结果表明:高温可以使大白菜脱春化;随着高温处理时间的延长,脱春化效果逐渐增强;种子脱春化效果比绿体脱春化效果好。基因组DNA甲基化水平检测结果表明:春化时大白菜植株发生去甲基化,脱春化处理可诱导其重新甲基化。
译  名：
Effects of Chinese Cabbage Devernalization and DNA Methylation Analysis before and after Devernalization
作  者：
WANG Xiao-li 1,ZHAO Yong 2,ZHANG Yao-wei 1*(1 College of Horticulture,Northeast Agricultural University,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Mionistory of Agriculture,Harbin 150030,Heilongjiang,China;2 Heilongjiang Province Cash Crop Technique Guiding Station,Harbin 150090,Heilongjiang,China)
关键词：
Chinese cabbage;Devernalization;DNA;Methylation
摘  要：
The spring Chinese cabbage〔Brassica campestris L.ssp.pekinensis(Lour) Olsson〕was easily bolting prematurely after having experienced low-temperature vernalization.To this phenomenon,this paper used F 1 hybrid'Yangchun'as testing material to study the green devernalization,seed devernalization in illumination culturing box,and green devernalization in greenhouse condition.The results showed that high-temperature could cause Chinese cabbage devernalization,and the result of devernalization turned better and better along with the time of hightemperature treatment prolonged.And the seed devernalization effect was better than that of green devernalization.The genomic DNA methylation level detection showed that vernalization would make the plant de-methylation,while devernalization treatment would induce re-methylation.

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