中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 重组鸡γ干扰素对柔嫩艾美球虫卵囊免疫鸡肠道免疫相关细胞数量的影响 中国兽医科学 2008,38 (8) 715-720
Position: Home > Articles > Regulatory effects of rChIFN-γ on the numbers of immune-associated cells in the intestinal mucosa of chickens vaccinated with Eimeria tenella oocysts Chinese Veterinary Science 2008,38 (8) 715-720

重组鸡γ干扰素对柔嫩艾美球虫卵囊免疫鸡肠道免疫相关细胞数量的影响

作  者：
YE Xiu-hua;蔡建平;WU Zhi-guang;佘锐萍;WANG Ming
单  位：
广东省农业科学院;中国农业大学动物医学院
关键词：
鸡;重组鸡γ干扰素;柔嫩艾美球虫;肠黏膜上皮内淋巴细胞;杯状细胞;肥大细胞
摘  要：
分别给7日龄和14日龄雏鸡免疫柔嫩艾美球虫孢子化卵囊并同时肌肉注射5000 U重组鸡γ干扰素(rChIFN-γ),21日龄再以5×104个同源柔嫩艾美球虫孢子化卵囊攻虫。分别取试验鸡各肠段制作组织切片,经HE和PAS染色后显微镜检查。发现21日龄时,rChIFN-γ加强免疫组鸡肠道黏膜上皮内淋巴细胞(IELs,9.8)和杯状细胞(GCs,26.2)数量显著高于免疫对照组(8.2、24.9);28日龄时,加强免疫组的IELs和GCs数量进一步增多(13.5、34.1)并显著高于免疫对照组(11.8、26.9)。用改良甲苯胺蓝组织化学染色法检测发现,rChIFN-γ可增加十二指肠、空肠、回肠和盲肠黏膜上皮肥大细胞(MCs)数量。结果提示,rChIFN-γ可有效刺激试验鸡肠道黏膜IELs、GCs和MCs的分化与增殖,具有增强肠道黏膜非特异性免疫的作用。
译  名：
Regulatory effects of rChIFN-γ on the numbers of immune-associated cells in the intestinal mucosa of chickens vaccinated with Eimeria tenella oocysts
作  者：
YE Xiu-hua1,2,CAI Jian-ping1,WU Zhi-guang2,IYAS2,SHE Rui-ping2,WANG Ming2 (1.Institute of Veterinary Medicine,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;2.College of Veterinary Medicine,China Agricultural University,Beijing 100094,China)
关键词：
chicken;rChIFN-γ;Eimeria tenella;intraepithelial lymphocyte;goblet cell;mast cell
摘  要：
Chickens were vaccinated with Eimeria tenella sporulated oocysts on day-old 7 and 14 respectively and injected simultaneously with 5 000 U recombinant chicken interferon gamma(rChIFN-γ).Then,the chickens were challenged with 5×104 homologous E.tenella sporulated oocysts on day-old 21 respectively,and their duodenum,caecum,jejunum and ileum were sampled for detecting the numbers of intestinal intraepithelial lymphocytes(IELs),goblet cells(GCs) and mast cells(MCs) in the intestinal mucosa by HE and PAS methods.The numbers of IELs and GCs in the chickens injected with rChIFN-γ were significantly more than those of the control chickens immunized only with E.tenella oocysts.The numbers of MCs in duodenum,caecum,jejunum and ileum of the chickens simultaneously injected with rChIFN-γ were also more than those of the controls vaccinated only with oocysts.These results showed that rChIFN-γ could stimulate the proliferation and differentiation of these immune-associated cells in the intestinal mucosa of chickens and boost the innate immunity against coccidiosis.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46