当前位置: 首页 > 文章 > 蝴蝶兰叶片诱导类原球茎的研究 湖北农业科学 2010,49 (5) 1033-1034
Position: Home > Articles > Study on Protocorn-like Body Induced from the Leaves of Phalaenopsis amabilis Hubei Agricultural Sciences 2010,49 (5) 1033-1034

蝴蝶兰叶片诱导类原球茎的研究

作  者:
张雯頩;张晓波;潘刚;刘宪虎
单  位:
浙江大学农业与生物技术学院;延边大学农学院
关键词:
蝴蝶兰;叶片;类原球茎
摘  要:
以14个蝴蝶兰(Phalaenopsis amabilis)品种无菌苗叶片为外植体诱导类原球茎(Protocorn-likebody,PLB)。结果表明,在培养基1/2MS+3mg/L TDZ+20g/L蔗糖+3g/L植物凝胶上,14个蝴蝶兰品种的叶片均能诱导出类原球茎,其中10个品种的诱导率高于20%,尤以777最高,为51%;最低的为788,仅有1%。试验建立了一套成本低廉、成分简单、重复性好、也适合于蝴蝶兰类原球茎的继代增殖培养技术体系,为蝴蝶兰的快速发展创造了条件。
译  名:
Study on Protocorn-like Body Induced from the Leaves of Phalaenopsis amabilis
作  者:
ZHANG Wen-ping1,ZHANG Xiao-bo2,PAN Gang2,LIU Xian-hu1 (1. College of Agriculture, Yanbian University, Longjing 133400, Jilin, China; 2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China)
关键词:
Phalaenopsis amabilis; leaf; protocorn-like body
摘  要:
The protocorn-like body was induced from leaves of fourteen varieties Phalaenopsis amabilis species by the same media. The results showed that the suitable medium for protocorn-like body inducement from leaves of fourteen varieties of P. amabilis was 1 / 2MS + 3mg / L TDZ + 20g / L sucrose + 3g / L phytagel. The induction rates of fourteen varieties were: V31:32%,8106:33%,729:46%,777:51%,604:21%,602-3:9%,791:28%,8169:24%,735:31%,607:21%,771:4%,608:38%, 728-3:18%,788:1%. The medium was low-cost,simple composition and had good reproducibility. And this medium was also suitable for propagation of the protocorn-like body.

相似文章

计量
文章访问数: 17
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊