译 名：
Influencing Factors of Protein Two-dimensional Electrophoresis in Cassava Leaf
作 者：
AN Feifei1) ZHANG Zhenwen1) ZHENG Yongqing1) LI Genghu2)(1 Tropical Crops Genetic Resources Institute,CATAS,Danzhou,Hainan 571737; 2 College of Agronomy,Hainan University,Haikou,Hainan 570228)
关键词：
cassava leaf;2-dimensional electrophoresis(2-DE);immobilized pH gradients(IPG) strip
摘 要：
South China 205(SC105) was adopted in this experiment to compare different effects of two-dimensional electrophoresis(2-DE) gels.Phenol extraction method was used to extract proteins.The results showed that immobilized pH gradients(IPG) strip with pH 4~7 can improve protein resolution in certain range.Sample loading of 200 mg produced better 2-DE gel image.The results not only provides techniques for getting a good 2-DE profile of cassava leaf,also for tuberous root,stem and other reproduction organ.
