当前位置: 首页 > 文章 > 海芋根状茎的离体培养与植株再生 北方园艺 2007,2007 (5) 216-217
Position: Home > Articles > In Vitro Culture and Plantlet Regeneration with Rhizome of Alocasia macrorrhiza Northern Horticulture 2007,2007 (5) 216-217

海芋根状茎的离体培养与植株再生

作  者:
杨翠芹;刘运权;方颖;秦耀国;刘伟
单  位:
四川农业大学农学院;华南农业大学生命科学学院;四川农业大学林学园艺学院
关键词:
海芋;离体培养;愈伤增殖;分化
摘  要:
以海芋的根状茎为外植体,探索了不同的生长调节剂配比对愈伤组织诱导、增殖与分化的影响。结果表明:含TDZ0.5mg/L的MS培养基上愈伤诱导率最高,达到53.3%;4mg/LBA+0.1mg/LNAA生长调节剂浓度有利于愈伤组织的增殖;附加1mg/LBA+0.05mg/LNAA的培养基上芽分化率最高;下表面切除0.1~0.2cm有利于愈伤组织增殖与分化。不定芽在无生长调节剂的1/2MS培养基中都能生根。
译  名:
In Vitro Culture and Plantlet Regeneration with Rhizome of Alocasia macrorrhiza
作  者:
YANG Cui-qin1,2,LIU Yun-quan2,FANG Ying2,QIN Yao-guo3,LIU Wei2(1.College of Agriculture,Sichuan Agricultural University,Yaan 625014;2.College of Life Science,South China Agricultural University,Guangzhou 510642;3.College of Forestry and Horticulture,Sichuan Agricultural University,Yaan 625014)
关键词:
Alocasia macrorrhiza;In vitro culture;Callus proliferation;Differentiation
摘  要:
Effects of different growth regulators combination on callus inducement,proliferation and differentiation were studied with rhizomes of Alocasia macrorrhiza as explants.Results showed that callus inducement rate was maximum and attained 53.3% in MS culture medium containing 0.5mg/L TDZ;It was advantageous for callus to proliferate with 4mg/L BA+0.1 mg/L NAA;Explants possessed the highest differentiation rate in culture medium with 1mg/L BA+0.05mg/L NAA.Callus excised 0.1~0.2cm thick in below surface was prone to proliferate and differentiat.When shoots were inserted into culture medium of 1/2MS,all of them produced roots.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊