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Position: Home > Articles > Detection of Resistant Phenotype of High-level Gentamicin-resistant Enterococcus from Fuzhou Zoo Animals Progress in Veterinary Medicine 2012,33 (7) 122-125

福州动物园动物高水平耐庆大霉素肠球菌耐药表型检测

作  者:
陈小丽;罗小青;唐耀;王璜;洪海波;陈夏;俞道进
单  位:
福建农林大学动物科学学院;福州市动物园管理处
关键词:
肠球菌;庆大霉素高水平耐药;动物园动物;耐药表型
摘  要:
为了解福州动物园动物细菌耐药现状,选用肠球菌为指示菌,采用琼脂平板筛选法(ADSP法)筛选高水平耐庆大霉素肠球菌(HLGRE)。根据美国临床实验室标准化协会(CLSI)推荐的微量肉汤稀释法测定耐药菌株对6种抗菌药物的耐药性,用PCR法对分离出的不同来源的高水平耐氨基糖苷类肠球菌进行菌种鉴定。从动物园圈养野生动物共分离出78株HLGRE,不同动物来源的分离率不同,其中草食动物HLGRE分离率最低,占55.56%;肉食动物HLGRE分离率最高,占84.38%。药敏试验结果显示,不同动物来源的HLGRE均对红霉素和土霉素的耐药率最高,分别为100%和96.1%,对氨苄西林和万古霉素的耐药率最低。
译  名:
Detection of Resistant Phenotype of High-level Gentamicin-resistant Enterococcus from Fuzhou Zoo Animals
作  者:
CHEN Xiao-li1,LUO Xiao-qing2,TANG Yao1,WANG Huang1, HONG Hai-bo2,CHEN Xia2,YU Dao-jin2(1.Fuzhou Zoo,Fuzhou,Fujian,350012,China;2.College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian,350002,China)
关键词:
Enterococcus;high-level gentamicin-resistance;zoo animal;resistant phenotype
摘  要:
To explore the resistance of bacteria from Fuzhou animal zoo to antimicrobials,Enterococcus was used as directed bacteria and ADSP method was used to isolate high-level gentamicin-resistant Enterococus(HLGRE).A micro-broth-dilution method recommended by CLSI was used to test the susceptibility of Enterococcus to 6 kinds of antimicrobials.A PCR method was used to identify the HLGRE.The result showed that there were 78 strains of HLGRE isolated from a total 110 samples.The isolation ratio of HLGRE differed from different animals,which was lowest from herbivorous animals(55.56%) and highest form carnivorous animals(84.38%).The result of antimicrobial susceptibility test indicated that HLGRE from different animal sources showed highest resistant ratio to ERY and OTC,which was 100% and 96.1% respectively,and the resistant ratio was low to AMP and VAN.

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