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Position: Home > Articles > Clonging and Sequence Analysis of Porcine MBF1 Gene Hubei Agricultural Sciences 2015,54 (22) 5748-5751

猪MBF1基因的克隆与序列分析

作  者:
武华玉;程碧军;乔木;黄京书;刘贵生;彭先文;李良华;吴俊静;梅书棋
单  位:
湖北省畜牧兽医局;湖北省农业科学院农业经济技术研究所;湖北省农业科学院畜牧兽医研究所
关键词:
猪;MBF1基因;克隆;序列分析
摘  要:
克隆了MBF1基因875 bp的c DNA序列和1 919 bp的基因全序列(登录号:EF 625885)。序列分析表明,猪MBF1基因序列与人、小鼠的相似性分别为89%和88%,氨基酸序列与人和小鼠的相似性分别为99%和97%,猪MBF1基因包含5个外显子和4个内含子,内含子的剪接方式都符合"GT-AG"法则;组织表达谱分析表明MBF1基因在心、肝、脾、肾、子宫中表达量较高,在胃和小肠中低表达。
译  名:
Clonging and Sequence Analysis of Porcine MBF1 Gene
作  者:
WU Hua-yu;CHENG Bi-jun;QIAO Mu;HUANG Jing-shu;LIU Gui-sheng;PENG Xian-wen;LI Liang-hua;WU Jun-jing;MEI Shu-qi;Institute of Animal and Veterinary Science, Hubei Academy of Agricultural Sciences;Institute of Agriculture Economics and Technology, Hubei Academy of Agricultural Sciences;Animal Husbandry and Veterinary Bureau of Hubei Province;
关键词:
porcine;;MBF1 gene;;clone;;sequence analysis
摘  要:
In this study, 875 bp m RNA sequence and 1 919 bp genomic sequence(EF 625885)of porcine MBF1 gene(multiprotein bridging factor 1) were cloned. A multiple alignment of MBF1 sequences showed that porcine MBF1 sequence had 89% identity with that of human, 88% identity with that of mouse, Amino acid sequence analysis showed that MBF1 had 99% identity with that of human, 97% identity with that of mouse. Sequence analysis revealed that porcine MBF1 gene consists of 5 exons and 4 introns and the locations of splice donor/acceptor sites in all introns conform to "GT/AG" rule. RTPCR analysis showed that MBF1 gene was highly expressed in heart, liver, spleen, kidney, uterus, and lowly expressed in stomach and small intestine.

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