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Position: Home > Articles > AFLP and SSR molecular markers linked to Verticillium wilt resistance gene Ve in tomato Acta Phytopathologica Sinica 2011,41 (1) 80-84

番茄黄萎病抗病基因Ve的AFLP和SSR分子标记

作  者:
雷娜;李景富;康力功;王傲雪;许向阳
单  位:
东北农业大学园艺学院;哈尔滨市农业科学院
关键词:
番茄;黄萎病;AFLP分子标记;SSR分子标记
摘  要:
本研究以番茄抗病品种05046与感病品种051355配制杂交组合,接种鉴定F1代及F2代分离群体的黄萎病发生情况,结果表明,番茄黄萎病属单基因显性遗传。用545对AFLP引物和101对SSR引物对两个亲本、抗感池及F2代分离群体进行AFLP和SSR分析,得到3个与番茄抗黄萎病基因Ve连锁的AFLP标记和1个SSR标记,分别是E66M 84-A、E78M 84-D、E66M 40-A和SSR599,与抗病基因Ve的连锁遗传距离分别为10.3、14.2、30.5和12.5 cM。
译  名:
AFLP and SSR molecular markers linked to Verticillium wilt resistance gene Ve in tomato
作  者:
LEI Na2,LI Jing-fu1,KANG Li-gong1,WANG Ao-xue1,XU Xiang-yang1 (1College of horticulture,Northeast Agricultural University,Harbin 150030,China;2Harbin Academy of Agricultural Science,Harbin 150070,China)
关键词:
tomato;Verticillium wilt;AFLP molecular markers;SSR molecular markers
摘  要:
A cross between Verticillium wilt resistant tomato variety 05046 and susceptible variety 051355 was made for mapping Verticillium wilt resistance gene(s).Through inoculation test on its F1 and F2 progeny,it was proved that Verticillium wilt resistance gene was a single recessive gene.With 545 AFLP primer combinations and 101 SSR primer combinations,AFLP and SSR analysis was performed on two parents,their F2 resis-tant and susceptible bulks and F2 progeny.Three new AFLP markers and one new SSR marker linked to the Verticillium wilt resistance gene-Ve gene were identified in the study.Three AFLP markers E66M84-A,E78M84-D and E66M40-A were mapped at 10.3 cM,14.2 cM and 30.5 cM genetic distance from the Ve gene respectively.The marker SSR599 was 12.5 cM away from the Ve gene.

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