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当前位置: 首页 > 文章 > 牛分支杆菌MPB70和CFP10-ESAT6基因的表达与检测 动物医学进展 2009,30 (5) 13-17
Position: Home > Articles > Expression and Detection of MPB70 and CFP10-ESAT6 Genes from Mycobacterium bovis Progress in Veterinary Medicine 2009,30 (5) 13-17

牛分支杆菌MPB70和CFP10-ESAT6基因的表达与检测

作  者：
薛梅;林祥梅;余多慰;李超;韩雪清
单  位：
中国检验检疫科学研究院;南京师范大学生命科学院
关键词：
牛分支杆菌;MPB70;CFP10-ESAT6;表达;酶联免疫吸附试验
摘  要：
以牛分支杆菌染色体DNA为模板,分别以MPB70、CFP10-ESAT6融合蛋白基因特异性引物进行PCR扩增,获得约600 bp的DNA片段,并将其克隆入PQE30质粒,构建出原核表达载体PQE30-MPB70,PQE30-CFP10-ESAT6。将质粒转化至感受态DH5α中,经IPTG诱导和SDS-PAGE分析,可见相应外源蛋白带。纯化蛋白后作为包被抗原建立ELISA方法,为进一步研究牛结核病诊断方法奠定基础。
译  名：
Expression and Detection of MPB70 and CFP10-ESAT6 Genes from Mycobacterium bovis
作  者：
XUE Mei1,LIN Xiang-mei2,YU Duo-wei1,LI Chao1,HAN Xue-qing2 (1.College of Nanjing Normal Universiy,Nanjing,Jiangsu,210046,China;2.Chinese Academy of Inspection & Quarantine,Beijing,100029,China)
关键词：
Mycobacterium bovis;MPB70;CFP10-ESAT6;expression;ELISA
摘  要：
The genes encoding MPB70 and CFP10-ESAT6 were amplified from chromosomal DNA of Mycobacterium bovis by using PCR technique,whose product was approximately 600 bp.PCR product was cloned into pQE30 vector,thus the recombinant vector pQE30-70 and pQE30-CFP10-ESAT6 were successfully constructed.The plasmid was transformed into the competence E.coli DH5α.The transformed bacteria were induced by IPTG and the lysates were loaded directly onto SDS-PAGE,which the protein was observed.The way of ELISA was found by using these proteins.These results could serve as a basis in further studies on the application of these M.bovis MPB70 gene and its development on diagnoses of tuberculosis.

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