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Position: Home > Articles > Studies on Different Methods of Parthenogenetic Activation on Porcine Zona-free Oocytes Chinese Agricultural Science Bulletin 2008,24 (4) 13-15

猪无透明带卵不同孤雌激活方法研究

作  者：
胡军和;屈雷;敬晓棋
单  位：
榆林学院生命科学学院
关键词：
猪;无透明带卵;孤雌激活;离子霉素
摘  要：
研究了离子霉素处理时间、处理浓度和不同胚胎基础培养基对猪无透明带卵孤雌激活的影响。结果表明:(1)10μM离子霉素处理5min、7min、10min的激活率64.81%、66.67%、68.75%差异不显著(P>0.05),但显著高于处理3min的激活率36.17%。(2)分别以10μM、15μM离子霉素处理10min的激活率68.18%、65.11%差异不显著(P>0.05),但显著高于5μM的激活率40.54%(P<0.05)。(3)分别以NCSU-23+4%BSA和TCM199+4%BSA作为基础培养基进行培养,其激活率68.51%、62.50%无显著差异(P>0.05)。试验结果表明:10μM离子霉素处理5min联合2mmol/L6-DMAP处理2h激活,以NCSU-23+4%BSA为胚胎基础培养基能有效提高猪无透明带卵孤雌激活的激活率。
译  名：
Studies on Different Methods of Parthenogenetic Activation on Porcine Zona-free Oocytes
作  者：
Hu Junhe1,2, Qu Lei1,2, Jing Xiaoqi 1,2 (1 Department of Life Science , Yu Lin College, Yu Lin 719000; 2 Centre of Shanbei White Cashmere goats Engineering and Technology, Yu Lin 719000)
关键词：
porcine, parthenogenetic, zona-free oocytes, ionomycin
摘  要：
The effects of ionomycin dealing time, ionomycin dealing density, different basic embryo culture mediums on parthenogenetic activation of porcine zona-free oocytes were studied. The results are as follows: (1) The activation rate with 10 μM ionomycin dealing 5 min(64.81%) was not significantly different from that of dealing 7 min(66.67%), 10 min(68.75%), but significantly higher than 3 min(36.17%). (2) The activation rate of ionomycin dealing 10 min with 10μM(68.18%) was not significantly different from that with 15μM (65.11%), but significantly higher than that with 5μM (40.54%). (3) The activation rate of using NCSU-23+4%BSA and TCM-199+4%BSA as embryo basic culture mediums was not significantly different(68.51%, 62.50%). The results of this experiment showed that 10 μM ionomycin dealing 5 min in combination with using NCSU-23+4%BSA can effectively activate porcine zona-free oocytes.

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