单 位：
莱州明波水产有限公司;农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所
关键词：
纳米探针;神经坏死病毒;表面增强拉曼散射
摘 要：
为了快速检测鱼类神经坏死病毒,本研究设计了病毒特异性的发夹型探针,经硫醇修饰后与纳米银溶胶结合,制备了鱼类神经坏死病毒纳米探针(Ag-NNV),并使用透射电镜、多功能酶标仪、表面增强拉曼光谱仪等对纳米探针Ag-NNV的表征及发夹型探针在纳米银粒子表面的覆盖率进行了测定和分析。结果表明,裸露的纳米银粒子为球形,平均直径50 nm;制备的纳米探针颗粒为球形,平均直径90 nm。纳米银粒子和纳米探针在溶液中均具有良好的分散性。平均每个纳米银粒子表面结合发夹型探针约100条,单位表面积的探针覆盖量为0.56 pmol/cm2。该纳米探针制备简便,适合用于表面增强拉曼散射法对鱼类神经坏死病毒进行快速检测。
译 名：
Production and Characterization of Silver Nanoprobe for Detection of Fish Nervous Necrosis Virus
作 者：
Su Zidan;Shi Chengyin;Liu Jiangchun;Wang Shengqiang;Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;College of Fisheries and Life Science,Shanghai Ocean University;Laizhou Mingbo Aquatic Company Ltd.;
关键词：
nanoprobe;;nervous necrosis virus;;surface-enhanced Raman scattering(SERS)
摘 要：
A nanoprobe(Ag-NNV)was prepared and characterized for detecting fish nervous necrosis virus. The morphology and spectroscopic properties of Ag-NNV nanoprobe were evaluated by transmission electron microscopy,automatic microplate reader and surface-enhanced Raman microscope. The results showed that the Ag-NNV nanoprobe was sphere-shaped with average diameter of 90 nm and dispersed well in the solution. The number of hairpin probe attached on a silver nanoparticle was estimated to be about 100 and the coverage density was 0.56pmol/cm2. With easy preparation,the Ag-NNV nanoprobe can be used for quick detection of fish nervous necrosis virus by surface enhanced Raman scattering.
