单 位：
天津大学药物科学与技术学院;天津红日药业股份有限公司
摘 要：
研究了对枫香叶中的挥发油成分的抗炎活性以及凝血作用,并考察了其挥发油成分。实验通过建立BEAS-2B人肺上皮细胞炎症模型,加入地塞米松+TNF-α诱导细胞阳性对照组(Dex)、枫香叶挥发油+TNF-α诱导细胞实验组,检测IL-6蛋白的表达量,评价挥发油的抗炎效果。采用体外血浆复钙实验,考察枫香叶挥发油对大鼠血浆的促凝作用。结合气相与质谱联用技术(GC-MS)对枫香叶挥发油进行了结构鉴定和分析。证实了枫香叶挥发油确实具有良好的抗炎及凝血作用,鉴定了挥发油中存在26种化学成分,主要成分为:α-蒎烯、β-蒎烯、柠檬烯、β-石竹烯等,其中10种化学成分为首次报道。从而为枫香叶这种具有保健、食品、药材用途的天然产物的深入开发奠定了基础。
译 名：
Pharmacological activity and chemical composition of Liquidambar formosana leaves volatile oil
作 者：
YANG Zhao-jun 1,ZHANG Yun-hui 1,FENG Jing 1,YAO Xiao-qing 2,JIN Xing-hua *(1.School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China;2.Tianjin Chase Sun Pharmaceutical Co.,Ltd.,Tianjin 301700,China)
关键词：
Liquidambar formosana leaves;volatile oil;anti-inflammatory;blood coagulation;identification
摘 要：
The Human bronchial epithelial(BEAS-2B) cells were pretreated with dexamethasone or essential oils prior to TNF-α stimulation.The anti-inflammatory activities were evaluated by the IL-6 release level.Lasma recalcification experiments were conducted to investigate the blood coagulation effect of the volatile oils in vitro.As a result,GC-MS method for analyzing the anti-inflammatory and blood coagulation activities of volatile oils from Liquidambar formosana was established.26 chemical compounds were identified by comparing with mass spectrum databases and literature data.Main ingredients included α-pinene,β-pinene,limonene,β-caryophyllene.A total of 10 compounds were reported for the first time.Thus the research laid the foundation for further development of Liquidambar formosana leaves which can be used in health care,food,medicine products.
