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Position: Home > Articles > Aseptic sowing and tissue culture of Cymbidium hybridum Hunan Forestry Science & Technology 2012,39 (3) 26-29

大花蕙兰无菌播种与组培快繁技术研究

作  者:
李茂娟;谭柏韬;邓少华;王华龙
单  位:
郴州市林业科学研究所
关键词:
大花蕙兰;无菌播种;组培快繁;原球茎;交替培养
摘  要:
对大花蕙兰的无菌播种及组培快繁技术进行了相关研究。结果表明:大花蕙兰无菌播种采用0.1%升汞溶液灭菌10 min能取得较好的效果;改良MS+6-BA1.0 mg/L+NAA 0.5 mg/L培养基对假鳞茎诱导原球茎效果较好,其原球茎诱导率可达86%;原球茎增殖的最佳培养基为改良MS+NAA 0.5 mg/L,此配方对大花蕙兰的壮苗生根同样合适;采用固、液培养基交替培养(振荡转数100~110 r/min)能有效地促进原球茎增殖;移栽基质配比为腐殖土∶棉籽壳=1∶1时,移栽苗成活率可达95%。
译  名:
Aseptic sowing and tissue culture of Cymbidium hybridum
作  者:
LI Maojuan,TAN Baitao,DENG Shaohua,WANG Hualong(Forestry Institute of Chenzhou City,Chenzhou 423000,China)
关键词:
Cymbidium hybridum;aseptic sowing;tissue culture;protocorm-like body(PLB);alternation culture
摘  要:
The aseptic sowing and tissue culture of Cymbidium hybridum were studied for rapid propagation of C.hybridum.The results showed that the disinfection treatment with 0.1%HgCl2 solution for 10 min was most suitable for aseptic sowing.The suitable medium for protocorm-like body(PLB) induction was modified MS +6-BA 1.0 mg/L+NAA 0.5 mg/L,the inductive rate reached 86%.The optimum medium for PLB propagation was modified MS +NAA 0.5 mg/L,which was also suitable for root growing.Liquid and solid medium alternation culture with shaking of 100~110 r/min could effectively promote the PLB propagation.The suitable transplanting medium was humus soil ∶ cotton seed hull=1 ∶ 1,the survival rate of transplanting seedlings reached 95%.

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