NaN3 Mutagenesis on Effects of Antioxidant Systems of Seed Germination and Seedling of Red Bean

LIU Jianxia;SU Qian;ZHOU Liqing;BAI Jing;ZHANG Rui;College of Life Science,Datong University;

sodium azide(NaN_3);;mutagenesis;;rice bean;;germination rate;;antioxidant system

Sodium azide(NaN_3)mutagenesis is an important way to obtain gene-level variants,understanding the basic conditions such as the treatment time and concentration of sodium azide is the precondition for mutagenesis breeding.In this study,we treated rice bean seeds by sodium azide solution on the concentrations of 0,0.2,0.6,1.0,1.4 and 1.8 mmol/L(solvent for phosphate buffer solution,pH=3)with treatment time of 16 h,20 hand 24 hrespectively.Though testing and analyzing germination rate and relative germination rate,we tested the optimum mutagenic dose concentration(half lethal dose LD 50)and suitable time of mutation.In addition,superoxide dismutase(SOD),sodium peroxidase(POD)and catalase(CAT)activity of the seedlings of rice bean M1 generation were detected,explore sodium azide effects on antioxidant system of rice bean seedlings.The results showed that the semi-lethal concentration of sodium azide on rice bean was 1.0 mmol/L and the time was 20 h.The activities of SOD and POD in the seedlings of rice bean were promoted by low concentration and inhibited by high concentration.While the CAT activity of red bean seedlings was always promoted.When the concentration of sodium azide was 1.0 mmol/L,the SOD activity of rice bean seedlings was the highest,while the concentration was 0.6 mmol/L,POD activity was the highest.