Determination of three major quality parameters of rapeseed with near infrared analyzer NYDL-3000

ZHI Wen-liang1,XIN Xiao-yang1,CUI Jian-min1,HU Sheng-wu1,ZHANG Wen2,LI Pei-wu2(1.College of Agronomy,Northwest Agriculture﹠Forest University,Yangling 712100,China; 2.Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Wuhan 430062,China)

NYDL-3000;Near infrared reflectance spectroscopy(NIRS);Rapeseed;Erucic acid;Total glucosinolates;Oil content

A near infrared reflectance spectrometer(NIRS) NYDL-3000 was designed by Oil Crops Research Institute of Chinese Academy of Agricultural Sciences.This research compared NYDL-3000 to traditional chemical methods in measuring three quality parameters of rapeseed including oil content,total glucosinolates and erucic acid.Oil content from NYDL-3000 was also compared to those from nuclear magnetic resonance spectrometer PC-120 and MATRIX-I Bruker Fourier transform NIR spectrometer.Coefficient of cross validation of oil content,total glucosinolates and erucic acid were 0.954 3,0.985 3 and 0.982 3;and RMSECV(root mean square error of cross validation) were 0.046 9,0.419 8 and 0.160 4 between NIRS and chemical methods.No obvious difference was found in cross validation between the two methods.External validation revealed that coefficient of correlation between NIRS and chemical methods were 0.837 6 and 0.951 7 for total glucosinolates and oil content.The compliance rate of erucic acid was 99.12% between NIRS and chemical method.No significant difference was found between the results of NIRS and chemical methods after tests of cross validation and external validation.Compared to other two spectrometers(as PC-120 and MATRIX-I),oil content results of NYDL-3000 respectively had coefficients of 0.956 6 and 0.901 5,and the RMSECV of 0.78%and 3.06%.It could be concluded that the NIRS NYDL-3000 analyzer was suitable for oil content,glucosinolates and erucic acid measurments on rapeseed breeding lines.