当前位置: 首页 > 文章 > 地黄连作障碍机制分析 中国农学通报 2012,28 (31) 227-230
Position: Home > Articles > Mechanism Analysis of Continuous Cropping Obstacle of Rehmannia glutinosa Chinese Agricultural Science Bulletin 2012,28 (31) 227-230

地黄连作障碍机制分析

作  者:
郭利川;刘常宏;左广胜;薛雅蓉;双惊雷
单  位:
南京大学生命科学学院;北京中龙创科技有限公司
关键词:
地黄;连作障碍;化感细菌
摘  要:
研究采用土壤微生物平板分离技术和16S rRNA基因序列分析技术,旨在研究化感细菌与地黄生长的关系,阐明引起地黄连作障碍的原因。从地黄连作1年的土壤中分离到19株细菌,其中2株细菌为恶臭假单胞菌(Pseudomonas putida DH03)和河生肠杆菌(Enterobacter amnigenus DH05);DH03菌株和DH05菌株不侵染地黄叶片,但其代谢产物对地黄叶片及块根有毒害作用,为典型的化感细菌;而地黄块根成分对DH03菌株和DH05菌株的生长有促进作用。根据化感细菌与地黄的相互作用,提出了引起地黄连作障碍的化感细菌作用假说,化感细菌的化感作用是引起地黄连作障碍的重要原因之一。
译  名:
Mechanism Analysis of Continuous Cropping Obstacle of Rehmannia glutinosa
作  者:
Guo Lichuan 1,Liu Changhong 1,Zuo Guangsheng 2,Xue Yarong 1,Shuang Jinglei 1(1 School of Life Science,Nanjing University,Nanjing 210093;2 Beijing Wlong Technology Co.Ltd,Beijing 100071)
关键词:
Rehmannia glutinosa;continue cropping problem;allelopathic bacteria
摘  要:
This study aimed to study the relationship between allelopathic bacteria and Rehmannia glutinosa growth,and clarify the causes of R.glutinosa continuous cropping cbstacle by using soil microbial plate isolation technique and 16S rRNA gene analysis method.From 1 year R.glutinosa replanted soil,19 strains of bacteria were extracted,two of which were identified to be Pseudomonas putida DH03 and Enterobacter amnigenus DH05.Strain DH03 and strain DH05 didn ' t infect R.glutinosa leaves.However,their metabolites had toxic effects on both leaves and tubes of R.glutinosa,and were typical allelopathic bacteria.Meanwhile,constitutes of R.glutinosa had a positive effect on the growth of strain DH03 and strain DH05.Based on the interactive effects between allelopathic bacteria and R.glutinosa,the hypothesis was proposed on the allelopathic bacterial effect which had caused the R.glutinosa continuous cropping cbstacle.Allelopathy of allelopathic bacteria was one of the main factors that had caused R.glutinosa continuous cropping obstacle.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊