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Position: Home > Articles > Cause and Prevention of Vitrifiation Shoots in Watermelon Journal of Shanxi Agricultural Sciences 2004,32 (3) 43-46

西瓜试管玻璃苗的成因及防止

作  者:
李竹梅;王果萍
单  位:
山西省农业生物技术研究中心;山西省农业种子总站
关键词:
组织培养;西瓜;玻璃苗;玻璃化现象
摘  要:
试管苗玻璃化现象是西瓜基因转化过程中试管苗继代培养的一大障碍。采用白炽灯光源,在2000~4000lx光照强度下光照12~14h,在培养基中提高糖和琼脂的浓度,添加适量的钙离子,细胞分裂素6-BA含量不超过6mg/L,生长素IAA用量控制在0.5mg/L,对克服西瓜试管苗玻璃化现象有明显的效果。
译  名:
Cause and Prevention of Vitrifiation Shoots in Watermelon
作  者:
LI Zhu-mei~1, WANG Guo-ping~2 (1.Shanxi Seeds Station,Taiyuan 030001,China; 2.Shanxi Center of Agro Biotechnology,Taiyuan 030031,China)
关键词:
Tissue culture;Watermelon;Vitrifiation shoots;Vitrifiation phenomenon
摘  要:
The phenomenon of vitrifiation shoots in tube regeneration culture of watermelon is a main obstacle in gene transformation.Adopting artifical light of 2 000~4 000 lx for 12~14 h,increasing concentration of sucrose and agar in the culture medium,adding proper calcium ion,and controlling the content of 6-BA at the level of less than 6mg/L and IAA at 0.5mg/L,could markedly prevent the occurrence of vitrifiation shoots during the watermelon tissue culture?

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