中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 星斑川鲽、黄盖鲽和石鲽线粒体基因Cytb和COⅠ片段序列的比较研究 水产科学 2016,35 (6) 625-632
Position: Home > Articles > Comparative Analysis of Mitochondrial Cytb and COⅠ Sequences of Flounders Platichthys stellatus,Pseudopleuronectes yokohamae and Kareius bicoloratus Fisheries Science 2016,35 (6) 625-632

星斑川鲽、黄盖鲽和石鲽线粒体基因Cytb和COⅠ片段序列的比较研究

作  者：
李青;王波;郑风荣;刘洪展;郭湘云;关洪斌
单  位：
山东大学海洋学院;国家海洋局第一海洋研究所
关键词：
星斑川鲽;黄盖鲽;石鲽;线粒体DNA
摘  要：
星斑川鲽、钝吻黄盖鲽和石鲽是我国北方重要的经济海水鱼类。为探究这3种鲽科鱼类间的亲缘关系及遗传信息,本文采用了PCR扩增和直接测序的技术,对这3种鲽科鱼类各24尾个体的线粒体基因Cytb和COⅠ进行初步研究。测序得到的序列经人工处理校正后分析,其中Cytb基因片段可用长度为358bp,包含58个变异位点,20个转换位点,3个颠换位点;COⅠ基因片段可用长度为559bp,包含变异位点56个,转换位点18个,颠换位点3个;Cytb基因片段和COⅠ基因片段均表明黄盖鲽和石鲽具有较高的单倍型多样性与较低的核苷酸多样性,而星斑川鲽具有较低的单倍型多样性和较低的核苷酸多样性。基于Cytb和COⅠ序列的两种系统发育树(邻接法和不加权对群法)均表明3种鲽科鱼类中星斑川鲽与石鲽亲缘关系更近,与黄盖鲽相对较远。鲽科鱼类物种遗传多样性及遗传结构的分析可以为鱼类的保护和管理提供重要的理论依据,为保障我国鲽科鱼类养殖业的健康可持续发展提供理论指导和科学依据。
译  名：
Comparative Analysis of Mitochondrial Cytb and COⅠ Sequences of Flounders Platichthys stellatus,Pseudopleuronectes yokohamae and Kareius bicoloratus
作  者：
LI Qing;WANG Bo;ZHENG Fengrong;LIU Hongzhan;GUO Xiangyun;GUAN Hongbin;Marine College,Shandong University;First Institute of Oceanography,State Oceanic Administration;
关键词：
Platichthys stellatus;;Pseudopleuronectes yokohamae;;Kareius bicoloratus;;mtDNA
摘  要：
Flounders Platichthys stellatus,Pseudopleuronectes yokohamae and Kareius bicoloratus were economically important marine fish in north China.To evaluate the relationship among the three members in Pleuronectidae and genetic information,mitochondrial DNA cytochrome b gene(Cytb)and mitochondrial cytochrome c oxidase subunitⅠ gene(COⅠ)fragments of the three species(24individuals in each species)were preliminarily studied by PCR amplification and direct sequencing.By analyzing the sequence obtained by manual processing,we found that the Cytb gene fragment was 358 bp,which contained 58 variable sites,20 transitionsal pairs,and 3transversional pairs,while the available length of the COⅠ gene fragment was 559 bp,including 56 variable sites,18 transitionsal pairs and 3transversional pairs.Analysis of Cytb and COⅠ gene fragments revealed that both P.yokohamae and K.bicoloratus had a high haplotype diversity and low nucleotide diversity while P.stellatus had low haplotype diversity and low nucleotide diversity.Two kinds of phylogeny trees(Neighbor-Joining Tree and UPGMA tree)based on cytb and COⅠ sequences showed that P.stellatus and K.bicoloratusis were closer than P.yokohamae in genetic relationship.Analysis of genetic diversity and genetic structure can provide an important theoretical basis for the conservation and management of fish,and provide theoretical guidance and scientific basis for the healthy and sustainable development of pleuronectid aquaculture.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46