Position: Home > Articles > Express Vector Construction of Trichinella Spiralis Newborn Larvae Stage-specific cDNAs
Journal of Tarim University
2002,14
(3)
1-4
旋毛虫新生幼虫期特异性基因糖蛋白表达载体的构建
作 者：
徐晓立;刘明远;卢强;任瑞文
单 位：
解放军军需大学动科系;塔里木农垦大学动物科技学院;广州军区连勤部军事医学研究所
关键词：
旋毛虫新生幼虫;期特异性基因;表达载体
摘 要：
根据旋毛虫新生幼虫 (NBL)期特异性全长cDNASSC1基因序列 ,PCR扩增目的基因 ,克隆入pMD18T后 ,根据其读码框架 ,克隆入表达载体pET2 8b ,构建其原核表达载体pET2 8b -SSC1。分别用SalI和XhoI、SmaI和HindIII双酶切 ;XbaI单酶切鉴定重组子 ,结果均与预期相符 ,进一步测序结果也表明重组载体读码框架正确 ,为进一步亚克隆和表达奠定基础
译 名：
Express Vector Construction of Trichinella Spiralis Newborn Larvae Stage-specific cDNAs
作 者：
Xu Xiaoli 1; Liu Mingyuan 2; Lu Qiang 2; Ren Ruiwen 3 (1 Institute of Animal Science and Technology ,Tarim University of Agricultural Reclamation,Alar, Xinjiang 843300; 2 Qurter Master Univercity of PLA,ChangChun 130062; 3 Military Medicine In
关键词：
NBL; stage-specific gene;express vector
摘 要：
Based on the open reading frame of full length SSC1 which has been identified as NBL stage-specific gene coding an glycoprotein,the forward PCR primer (AGGTCGACGTTGCAACATGCAAAAACG)with a Sal I enzyme site has been designed using the software DNAsis. Using the forward primer and T7 primer, the full length gene of SSC1 was amplified. After cloned in pMD18 T Vector and digested with the enzyme Sal I and Xho I,and digested pET28b with Sal I, Xho I and CIAP, the stage specific gene SSC1 was recombined with the T7 promoter-driven expression vector pET28b.The identified with Enzyme digested and sequenced results consistent with designed,which suggested it can be used as express vector.
导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
