作 者：
阮玲云;苏达龙;单书凯;李哲;李钊锋;胡开辉;孙淑静
摘 要：
银耳芽孢不仅可作为新型生物反应器生产外源药用蛋白,其多糖也具有重要药用价值,通过测定不同p H值、氮源、碳源、无机盐及营养饥饿等条件下银耳芽孢及胞内外多糖产量,探索各营养条件对银耳芽孢、多糖产量及分泌多糖能力的影响。结果表明,最适初始p H为5,碳源为麦芽糖,氮源为Na NO3,银耳芽孢和胞内外总多糖产量最高,与其它相同条件比较达到显著性差异,在以上单因素变化中,芽孢最高产量13.8 g/100m L,总多糖最高产量为145.31 mg/100m L。但蔗糖及NH4Cl有利于胞外多糖的产生。不同培养基成分实验结果表明,低氮及高无机盐不仅有利于芽孢的生长及多糖的产生,而且有利于多糖的分泌。
译 名：
Effects of Different Conditions on the Yield of Polysaccharides and Spores from Tremella fuciformis
作 者：
RUAN Ling-yun;SU Da-long;SHAN Shu-kai;LI Zhe;LI Zhao-feng;HU Kai-hui;SUN Shu-jing;College of Life Sciences, Fujian Agriculture and Forestry University;
关键词：
Tremella fuciformis;;fermentation;;nutritional condition;;polysaccharide
摘 要：
Tremella fuciformis spores were used as new type bioreactor to produce foreign medical protein. At the same time the polysaccharides of T. fuciformis spores had many medical functions for human. The yields of spores and polysaccharides of T. fuciformis were determined under different conditions including different p H value, nitrogen sources, carbon sources and other medium components for exploring the effects of all kinds of conditions on the yields of spores and polysaccharides. The results showed the optimum p H, carbon and nitrogen sources for producing the maximum polysaccharide yield were respectively p H 5,maltose and Na NO3. Among the change of single factor, the yields of spores and polysaccharides were highest respectively 13.8 g/100 m L and 145.31 mg/100 m L. The production of exopolysaccharides( EPS) was higher using sucrose as carbon source and NH4 Cl as nitrogen source. The low nitrogen content and high inorganic salts content were to benefit of spores production and polysaccharides, especially the production of EPS.
