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当前位置: 首页 > 文章 > 16种野豌豆属植物花粉形态的扫描电镜观察 草业学报 2015,24 (1) 107-114
Position: Home > Articles > Observation of pollen morphology of 16 Vicia accessions using scanning electron microscopy Acta Prataculturae Sinica 2015,24 (1) 107-114

16种野豌豆属植物花粉形态的扫描电镜观察

作  者：
刘鹏;马利超;王宇;刘志鹏
单  位：
草地农业生态系统国家重点实验室;兰州大学草地农业科技学院
关键词：
野豌豆属;花粉;形态;扫描电镜;观察
摘  要：
野豌豆属牧草适宜在高寒和高海拔地区栽培,是我国高原牧区重要的蛋白饲料来源。该属有190个物种,4个亚属:Vicia,Cracca,Ervum和Faba。本研究从中国、美国、土耳其和叙利亚等11个国家收集了16份野豌豆属牧草种质,利用扫描电镜对这16份材料的花粉形态进行观察。结果表明,野豌豆属植物花粉均以单粒形式存在,极面观为三裂圆形和三角形,赤道面观为扁圆形、长椭圆形和椭圆形。三沟萌发孔,沟长深入两端;极轴长为(9.60±1.52)~(30.40±2.07)μm,赤道轴长为(11.80±0.84)~(38.20±0.84)μm。花粉的表面纹饰呈3种类型:网状纹饰,脑状纹饰和疣状纹饰。网状纹饰根据外壁纹饰疏松程度不同,又可分为2种亚类型:粗网状纹饰和细网状纹饰。本研究较为系统地观察了16个野豌豆属牧草的花粉形态,探讨了其系统分类学特性,为揭示野豌豆属种质资源的遗传多样性奠定了基础。
译  名：
Observation of pollen morphology of 16 Vicia accessions using scanning electron microscopy
作  者：
LIU Peng;MA Lichao;WANG Yu;LIU Zhipeng;College of Pastoral Agriculture Science and Technology,Lanzhou University,State Key Laboratory of Grassland Agro-ecosystems;
关键词：
Vicia;;pollen;;morphology;;scanning electron microscopy;;observation
摘  要：
Plants of the genus Vicia are readily cultivated in alpine and high-altitude areas,such as the Tibetan plateau in China,where they are used as a source of protein.There are about 190 species in this genus,and four subgenera:Vicia,Cracca,Ervumand Faba.We studied 16 Vicia accessions sourced from China,USA,Turkey,Syria,and 7other countries.The pollen morphology of the 16 accessions was observed by scanning electron microscopy.Pollen of Viciais single-grained,with three lobes and a triangular outline in polar view.The pollen studied ranged in shape from prolate-spheroidal to prolate andoblate in equatorial view with three channel apertures reaching both poles.Polar axis length was(9.60±1.52)-(30.40±2.07)μm,and equatorial axis length was(11.80±0.84)-(38.20±0.84)μm.Three types of pollen surface ornamentation were observed:reticulate,brain-like and warty.Furthermore,depending on the degree of protrusion,the reticulation type can be divided into coarse and fine reticulation sub-types.In this study,the pollen morphologies of 16 Vicia accessions were observed in detail,and their taxonomic characteristics are discussed as a basis for categorising the diversity in the Vicia genus.

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