关键词：
热带螨;重组Blo t 21蛋白;气道炎症
摘 要：
为了建立热带螨重组变应原Blo t 21(rBlo t 21)诱导小鼠变态反应气道炎症模型,笔者利用从NCBI Gen Bank库里获得的热带螨Blo t 21基因序列,进行了序列优化与全基因合成,构建了原核表达载体PQE80LrBlo t 21,在大肠杆菌内诱导表达rBlo t 21,利用镍柱亲和层析纯化rBlo t 21蛋白。12只BALB/c小鼠随机分为对照组(PBS组)与诱导组(rBlo t 21组),每组6只,通过腹腔注射致敏与气道诱导后,分别通过酶联免疫吸附试验(ELISA)检测血清总IgE水平;鼠尾静脉注射变应原诱发应激性过敏反应;HE染色观察小鼠肺部炎症严重程度。本试验成功表达与纯化了重组热带螨变应原Blo t 21。利用纯化的rBlo t 21诱导小鼠变态反应气道炎症模型,与PBS组相比,rBlo t 21组的小鼠血清总IgE水平显著升高,肺部组织细胞侵润明显增加(P<0.05)。原核表达纯化的重组Blo t 21变应原能够诱导小鼠产生变态反应气道炎症。
译 名：
The Development of Airway Inflammation Murine Model Induced by Recombinant Blomia tropicalis Group 21 Allergen
作 者：
XIAO Zhengpan;LIU Jinying;WEI Shuangshuang;WANG Dayong;PEI Yechun;Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research,College of Animal Science and Technology,Institute of Tropical Agriculture and Forestry,Hainan University;College of Biological Science,Institute of Tropical Agriculture and Forestry,Hainan University;
单 位：
Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research,College of Animal Science and Technology,Institute of Tropical Agriculture and Forestry,Hainan University%College of Biological Science,Institute of Tropical Agriculture and Forestry,Hainan University
关键词：
Blomia tropicalis;;recombinant Blo t 21;;airway inflammation
摘 要：
An attempt was made to develop airway inflammation murine model induced by the recombinant Blo t21 allergen.The Blo t 21 gene sequences were obtained from NCBI Gen Bank,then optimized,synthesized and inserted into the prokaryotic expression vector PQE80 L.The recombinant Blo t 21 was expressed in E.coli bacteria,and purified by NI-NTA agarose.Twelve BALB/c mice were divided randomly into control group(PBS group,n=6) and rBlo t 21-induced group(rBlo t 21 group,n=6).After sensitization by intraperitoneal injection(i.p) and challenged by intratracheal instillation of rBlo t 21,the total serum IgE levels were measured by ELISA;the lungs were fixed and stained with haematoxylin and eosin(H&E).The recombinant Blo t 21 allergen was expressed successfully in E.coli and purified by Ni-NTA affinity chromatography.Furthermore,the airway inflammation murine model were induced by the recombinant Blo t 21 allergen.Compared with the control group(PBS group),the total serum IgE levels rose obviously in the rBlo t 21 group(p<0.005).Mice in the rBlo t 21 group showed inflammatory cell infiltration in airway by haematoxylin and eosin(H&E).The airway inflammation murine model was induced successfully by the recombinant Blo t 21 allergen.
