中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 氯霉素多克隆抗体的制备及其免疫学特性鉴定 中国农学通报 2006,22 (6) 5-9
Position: Home > Articles > Generation and Characterization of Immunological Traits of Polyclonal Antibody against Chloramphenicol Chinese Agricultural Science Bulletin 2006,22 (6) 5-9

氯霉素多克隆抗体的制备及其免疫学特性鉴定

作  者：
王自良;张海棠;王艳荣;刘俊伟;吴玉平;柴书军;张改平
单  位：
河南科技学院动物科学学院;河南省动物免疫学重点实验室
关键词：
氯霉素;半抗原;人工免疫原;多克隆抗体;免疫学特性
摘  要：
将氯霉素(CAP)进行化学修饰引入羧基活性基团,合成具有半抗原结构特征的氯霉素半琥珀酸酯(CAP-HS);采用混合酸酐(MA)法将CAP-HS与牛血清白蛋白(BSA)和卵清蛋白(OVA)偶联合成人工免疫原BSA-CAP-HS和包被原OVA-CAP-HS,用红外(IR)、紫外(UV)、凝胶电泳(SDS-PAGE)进行鉴定;用BSA-CAP-HS免疫新西兰白兔和SPF鸡制备多克隆抗体(pAb),间接ELISA测定其效价,阻断ELISA鉴定其敏感性,琼脂双扩散试验、WestGold膜杂交试验和交叉反应试验鉴定其特异性。结果表明,BSA-CAP-HS偶联成功,其分子结合比为15.6∶1,获得了高价、敏感、特异的CAPpAb,为CAP残留免疫学检测方法的建立奠定了基础。
译  名：
Generation and Characterization of Immunological Traits of Polyclonal Antibody against Chloramphenicol
作  者：
Wang Ziliang1, Zhang Haitang1, Wang Yanrong1, Liu Junwei1, Wu Yuping1, Chai Shujun2, Zhang Gaiping2 (1The Animal Science College, Henan Institute of Science and Technology, Xinxiang, Henan 453003; 2Henan Provincial Key Laboratory for Animal Immunology, Zhengzhou, Henan 450002)
关键词：
Chloramphenicol, Hapten, Artificial immunogen, Polyclonal antibody; Immunological traits
摘  要：
The active group carboxyl was introduced to Chloramphenicol(CAP) and formed CAP half succinaate (CAP-HS) which has hapten structure by chemical modification. The method of mixed anhydride (MA) was used to conjugate CAP-HS to BSA and obtained artificial immunogen BSA-CAP-HS, the coating antigen OVA-CAP-HS was obtained in the same way. IR, UV and SDS-PAGE were used to identify BSA-CAP-HS. New Zealand white rabbits and SPF chickens were immunized with BSA-CAP-HS,the titre of polyclonal antibody(pAb) was detected by indirect ELISA, the sensitivity of pAb was identified by blocking ELISA and the specificity of pAb was identified by test of agar bidirectional pervasion, WestGold immunochromatography and cross-reaction. The results showed that BSA-CAP-HS had been synthesized successfully and its conjugation ratio of CAP-HS to BSA was about 15.6∶1, the high-titer, sensitive and specific CAP pAb had been produced in sera of immunized New Zealand white rabbits and SPF chickens, which made it possible to establish immunoassay of CAP residues in feed and animal food.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46