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当前位置: 首页 > 文章 > CmMLO2:一个与甜瓜白粉病感病相关的新基因 园艺学报 2013,40 (3) 540-548
Position: Home > Articles > CmMLO2:A Novel Gene Closely Associated with the Powdery Mildew in Melon Acta Horticulturae Sinica 2013,40 (3) 540-548

CmMLO2:一个与甜瓜白粉病感病相关的新基因

作  者：
程鸿;孔维萍;何启伟;王晓巍
单  位：
山东省农业科学院蔬菜研究所;甘肃省农业科学院蔬菜研究所
关键词：
甜瓜;白粉病;MLO;基因表达;遗传转化
摘  要：
通过RT-PCR方法从甜瓜中克隆得到白粉病感病相关基因CmMLO2的cDNA序列,GenBank登录号为FJ713542。该基因ORF全长1713bp,可编码由570个氨基酸组成的蛋白质,具有7个跨膜螺旋结构,属于典型的跨膜蛋白。RT-PCR结果表明,CmMLO2主要在甜瓜叶片中表达,具有组织特异性。在受到白粉病菌胁迫时CmMLO2表达显著上调,表明CmMLO2很有可能与白粉病发病有关。构建RNAi表达载体pFGC1008-CmMLO2后,利用叶盘转化法转化甜瓜,获得了PCR阳性植株,白粉病接种鉴定结果表明,转化植株对白粉病具有抗性,证明通过ihpRNAi敲除CmMLO2,可以获得对白粉病具有抗性的甜瓜材料。
译  名：
CmMLO2:A Novel Gene Closely Associated with the Powdery Mildew in Melon
作  者：
CHENG Hong 1,* ,KONG Wei-ping 1 ,HE Qi-wei 2 ,and WANG Xiao-wei 1 ( 1 Institute of Vegetable,Gansu Academy of Agricultural Science,Lanzhou Research Station of Horticultural Crop Biology and Germplasm Enhancement,Ministry of Agriculture,P. R. China,Lanzhou 730070,China; 2 Institute of Vegetable, Shandong Academy of Agricultural Science,Ji’nan 250100,China)
关键词：
melon;powdery mildew;MLO;gene expression;genetic transformation
摘  要：
The full-length cDNA sequence of the MLO gene was cloned via RACE-PCR from muskmelon(Cucumis melo L.),and was designated as CmMLO2(GenBank Accession No. FJ713542). The gene is 1713 bp long and encodes a 570-amino acid peptide with a seven-transmembrane domain topology,and is a typical transmembrane protein. Its expression pattern was analyzed using quantitative real-time PCR after the melon leaf was infected with powdery mildew. The results indicate that CmMLO2 is mainly expressed in melon leaves in a tissue-specific distribution. Moreover,CmMLO2 may play a crucial role in the pathogenesis of powdery mildew. After the efficient of dsRNA constructs were examined via pFGC1008-CmMLO2 were introduced into Agrobacterium tumefaciens and transformed into melon wild type with the methods of Agrobacterium-mediated leaf disc transformation. The material of broad-spectrum powdery mildew resistance against P. xanthii in muskmelon was obtained by ihpRNAi-mediated knock-down of CmMLO2.

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