译 名：
Inhibitory Effects of Inulae Radix Extracts against Sortase A Acitivity of Staphylococcus aureus
作 者：
TANG Fayin;LI Wenhua;DENG Xuming;College of Veterinary Medicine,Jilin University;College of Pharmaceutical Engineering,Henan University of Animal Husbandry and Economics;
关键词：
sortase A;;S. aureus;;Inulae Radix extract;;anti-infection
摘 要：
Bacterial resistance has become one of the most urgent global problems in this century,in which drug-resistant Staphylococcus aureus has attracted much attention. Sortase A is indispensable for the anchorage of virulence associated surface proteins,suggesting that this protein is an ideal target for developing anti-infective drugs. Sortase A activity inhibition assay,extracellular matrix adhesion assay,bacterial biofilm formation assay and bacterial invasive assay were performed to indentify sortase A inhibitor and its inhibitory effect against S. aureus virulence. We found that Inulae Radix extracts could significantly inhibit the activity of sortase A. Furthermore,treatment with Inulae Radix extracts significantly reduced the adhesion of S. aureus to extracellular matrix and the formation of bacterial biofilm. Additionally,the addition of Inulae Radix extracts into the co-culture system of S. aureus and host cells could significantly inhibit the invasion of bacteria to host cells. Our results indicated that Inulae Radix extracts could attenuate S. aureus virulence by targeting sortase A,suggesting that Inulae Radix extracts may represent a leading compound for S. aureus infection.
