Position: Home > Articles > Study on Swine Blood Fermentation Conditions of Bacillus A32
FOOD SCIENCE
2006,27
(4)
119-123
发酵猪血优良菌株A32发酵条件的研究
作 者：
方俊;卢向阳;邱业先;莫瑾;蒋红梅;马美湖;李宗军
单 位：
湖南农业大学生化与发酵工程实验室;仲凯农学院
关键词：
猪血发酵;菌株A32;正交试验
摘 要：
从农村个体屠宰场土壤中分离到发酵猪血优良菌株A32,为了进一步提高A32菌株发酵猪血的能力,通过单因素试验以及正交试验确定了A32最优培养基配方:豆粕粉2%、玉米粉3%、0.4mmol/LMn2+、Na2HPO4·12H2O0.4%、KH2PO40.03%、Na2CO30.01%、pH7.2;同时还研究了培养时间、摇瓶转速、接种量、温度对产酶的影响,试验表明培养时间48h,接种量3%、温度30℃组合有利于蛋白酶的产生,摇瓶转速190r/min较为理想。
译 名：
Study on Swine Blood Fermentation Conditions of Bacillus A32
作 者：
FANG Jun1,LU Xiang-yang1,*,QIU Ye-xian2,MO Jin1,JIANG Hong-mei1, MA Mei-hu1,LI Zong-jun1 (1.The Lab of Biochemistry and Fermentation Engineering, Hunan Agricultural University, Changsha 410128, China;2.Zhongkai Agricultural College, Guangzhou 510000, China)
关键词：
swine blood meal fermentation;strain A32;orthogonal experiments
摘 要：
Bacillus A32 has been isolated from the slaughtering house soil whose ability on fermenting swine blood was superior. To improve the ability on fermenting swine blood by A32, fermentation conditions were studied on various factors and orthogonal experiments. The optimum fermentation medium containing soybean powder 2%, cornmeal 3%, Na2HPO4·12H2O 0.4%, KH2PO4 0.03%, Na2CO3 0.01% and Mn2+ 0.4mmol/L with pH7.2. At the same time, the effects for producing protease such as time, rotation speed, inoculum size, temperature and metal ions were studied. The results showed that 48h, 190r/min, 2% inoculum size and 30℃ are optimum for producing protease.
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