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Position: Home > Articles > Primary epidemiological survey on the infection of hepatitis E virus in swine in Beijing suburbs Chinese Journal of Veterinary Medicine 2012,48 (6) 6-9

北京郊区猪戊型肝炎流行初步调查

作  者:
李靖;刘波;杨君敬;田德雨;凌勇;何诚
单  位:
中国农业大学动物医学院
关键词:
猪;戊型肝炎病毒;血清流行病学;逆转录聚合酶链式反应
摘  要:
用间接ELISA试剂盒对北京市郊区所采集的174份猪血清样品进行了抗体检测,结果显示,其抗体总阳性率达62.1%。针对戊型肝炎病毒(HEV)ORF2/ORF3重叠区设计了简并引物,采用巢式RT-PCR对随即采取的少量猪粪便样品进行了抗原检测。结果表明,部分粪便样品中存在HEV。
译  名:
Primary epidemiological survey on the infection of hepatitis E virus in swine in Beijing suburbs
作  者:
LI Jing,LIU Bo,YANG Jun-jing,TIAN De-yu,LING Yong,HE Cheng(College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)
关键词:
swine;hepatitis E virus;Serology;Reverse transcriptase polymerase chain reaction
摘  要:
To investigate hepatitis E virus(HEV) infection in pigs in Beijing suburbs,we collected 174 swine sera,and tested antibodies using ELISA kit.The results demonstrate that the total positive rate of anti-HEV antibodies was 62.1%.Responses in a nested RT-PCR assay to a pair of degenerated primers designed from ORF2/ORF3 overlapping region indicated presence of gene sequence of HEV in the stool samples.

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