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Position: Home > Articles > Establishment of TR-PCR Assay for Detection of Infectious Bursal Disease Virus in Chicken Carcases China Animal Health Inspection 2010,27 (4) 30-32

白条鸡传染性法氏囊病病毒RT-PCR检测方法的建立和初步应用

作  者:
杜瑞玲;李银聚;张春杰;程相朝;吴庭才
单  位:
中牟县质量技术监督局;河南科技大学动物科技学院
关键词:
白条鸡;传染性法氏囊病病毒;RT-PCR;检测
摘  要:
设计了一对特异性引物,对具有临床症状、病理变化明显和AGP检测呈阳性的病鸡屠体的肺脏和鼻腔粘液进行RT-PCR扩增。结果显示,6个样品的肺脏全部扩增出特异性基因片段,阳性符合率为100%,鼻腔粘液有5例扩增出阳性基因片段,符合率为80%。将建立的RT-PCR检测方法用于市售白条鸡的检测,90只随机样品中有7只扩增出阳性片段,检出率为7.78%。本试验建立的RT-PCR检测方法为肉食品原料卫生的检验提供了新的技术手段。
译  名:
Establishment of TR-PCR Assay for Detection of Infectious Bursal Disease Virus in Chicken Carcases
作  者:
DU Rui-ling1,LI Yin-ju2,ZHANG Chun-jie2,CHENG Xiang-chao2,WU Ting-cai2(1.Zhongmou Bureau of Quality and Technical Supervision,zhongmou,Henan 451450,China;2.Animal Science and Technology College,Henan University of Science and Technology,Luoyang 471003,China)
关键词:
chicken carcases;infectious bursal disease virus ;RT-PCR;detection
摘  要:
A reverse transcription-polymerase chain reaction(RT-PCR) assay was developed by using primers specific to infectious bursal disease virus(IBDV).6 suspect cases of infectious bursal disease(IBD),which clinical manifestation,pathological changes and AGP were positive of chicken lungs and cavum nasi mucosal fluid were amplified using RT-PCR.The results showed that the 6 samples were all positive and the positive conformable probability of chicken lungs and cavum nasi mucosal fluid were 100% and 80%,respectively.7 samples were positive in the 90 chicken naked body collected from the market,and the detection rate is 7.78%.The developed of RT-PCR provide a new method to the accurate detect of IBDV in chicken naked body.

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